Zeiss HBO 100 Wartungshandbuch

Operating Manual Axio Scope.A1 Microscope for Routine and Entry-Level Research

INTRODUCTION Carl Zeiss Safety Guidelines Axio Scope.A1 10 M60-2-0007 e 05/08 The microscope Axio Scope.A1 and its genuine accessories may

OPERATION Carl Zeiss Operating and Functional Elements of Optional Components Axio Scope.A1 100 M60-2-0007 e 05/08 4.2.7 Upper Stand Part

OPERATION Axio Scope.A1 Operating and Functional Elements of Optional Components Carl Zeiss M60-2-0007 e 05/08 101 4.2.8 Upper Stand Part

OPERATION Carl Zeiss Operating and Functional Elements of Optional Components Axio Scope.A1 102 M60-2-0007 e 05/08 4.2.10 Sideport ISCP T6

OPERATION Axio Scope.A1 Operating and Functional Elements of Optional Components Carl Zeiss M60-2-0007 e 05/08 103 4.2.11 Reflector Slider

OPERATION Carl Zeiss Operating and Functional Elements of Optional Components Axio Scope.A1 104 M60-2-0007 e 05/08 Condenser 0.9/1.25 H The

OPERATION Axio Scope.A1 Operating and Functional Elements of Optional Components Carl Zeiss M60-2-0007 e 05/08 105 Binocular ergo tube / er

OPERATION Carl Zeiss Operating and Functional Elements of Optional Components Axio Scope.A1 106 M60-2-0007 e 05/08 Binocular photo tube (10

OPERATION Axio Scope.A1 Operating and Functional Elements of Optional Components Carl Zeiss M60-2-0007 e 05/08 107 Mechanical stage 75x50 −

OPERATION Carl Zeiss Operating and Functional Elements of Optional Components Axio Scope.A1 108 M60-2-0007 e 05/08 Diaphragm slider 14x40 m

MAINTENANCE, CHANGING OF FUSES AND SERVICE Axio Scope.A1 Maintenance Carl Zeiss M60-2-0007 e 05/08 109 5 MAINTENANCE, CHANGING OF FUSES AN

INTRODUCTION Axio Scope.A1 Safety Guidelines Carl Zeiss M60-2-0007 e 05/08 11 Gas discharge lamps, e.g. HBO 100, emit ultraviolet radiati

MAINTENANCE, CHANGING OF FUSES AND SERVICE Carl Zeiss Technical Service Axio Scope.A1 110 M60-2-0007 e 05/08 5.2 Technical Service 5.2.1

MAINTENANCE, CHANGING OF FUSES AND SERVICE Axio Scope.A1 Trouble Shooting Carl Zeiss M60-2-0007 e 05/08 111 5.3 Trouble Shooting Problem

MAINTENANCE, CHANGING OF FUSES AND SERVICE Carl Zeiss Trouble Shooting Axio Scope.A1 112 M60-2-0007 e 05/08 Problem Cause Fault clearance

MAINTENANCE, CHANGING OF FUSES AND SERVICE Axio Scope.A1 Trouble Shooting Carl Zeiss M60-2-0007 e 05/08 113 Problem Cause Fault clearance

MAINTENANCE, CHANGING OF FUSES AND SERVICE Carl Zeiss Service Axio Scope.A1 114 M60-2-0007 e 05/08 5.4 Service Service work on the mechani

ANNEX Axio Scope.A1 List of Abbreviations Carl Zeiss M60-2-0007 e 05/08 115 6 ANNEX 6.1 List of Abbreviations AC Alternating Current A

ANNEX Carl Zeiss List of Abbreviations Axio Scope.A1 116 M60-2-0007 e 05/08 R Right-hand (gear knob on the right-hand side of the mecha

ANNEX Axio Scope.A1 Subject Index Carl Zeiss M60-2-0007 e 05/08 117 6.2 Subject Index page A Achromatic illumination adapter ...

ANNEX Carl Zeiss Subject Index Axio Scope.A1 118 M60-2-0007 e 05/08 F Field diaphragm...

ANNEX Axio Scope.A1 Subject Index Carl Zeiss M60-2-0007 e 05/08 119 N Nosepiece...

INTRODUCTION Carl Zeiss Safety Guidelines Axio Scope.A1 12 M60-2-0007 e 05/08 Never look into the light beam of the illumination equipmen

ANNEX Carl Zeiss Subject Index Axio Scope.A1 120 M60-2-0007 e 05/08 Transmitted light ...

ANNEX Axio Scope.A1 Property Rights Carl Zeiss M60-2-0007 e 05/08 121 6.3 Property Rights The instruments, their components and the metho

INTRODUCTION Axio Scope.A1 Safety Guidelines Carl Zeiss M60-2-0007 e 05/08 13 Caution signs on lower stand part for HAL 100- and HAL 50-ill

INTRODUCTION Carl Zeiss Warranty Axio Scope.A1 14 M60-2-0007 e 05/08 1.2 Warranty The manufacturer guarantees that the instrument is free

INTRODUCTION Axio Scope.A1 Stand Variants Carl Zeiss M60-2-0007 e 05/08 15 1.3 Stand Variants The shipment includes five different upper s

INTRODUCTION Carl Zeiss Stand Variants Axio Scope.A1 16 M60-2-0007 e 05/08 Upper stand part Lower stand part Upper stand part Backli

INTRODUCTION Axio Scope.A1 Interface Diagram Carl Zeiss M60-2-0007 e 05/08 17 1.4 Interface Diagram The following figure shows a diagram o

INTRODUCTION Carl Zeiss Interface Diagram Axio Scope.A1 18 M60-2-0007 e 05/08 Fig. 1-3 Interface diagram (upper stand part FL/HBO and lo

INTRODUCTION Axio Scope.A1 Summary of the Controls and Functional Elements Carl Zeiss M60-2-0007 e 05/08 19 1.5 Summary of the Controls an

Carl Zeiss Copyright / Trademark Axio Scope.A1 2 M60-2-0007 e 05/08 A thorough knowledge of these instructions is mandatory in order to o

INTRODUCTION Carl Zeiss Summary of the Controls and Functional Elements Axio Scope.A1 20 M60-2-0007 e 05/08 Fig. 1-4 Summary of the cont

TECHNICAL MANUAL Axio Scope.A1 Intended Use Carl Zeiss M60-2-0007 e 05/08 21 2 TECHNICAL MANUAL 2.1 Intended Use The microscopes Axio Sc

TECHNICAL MANUAL Carl Zeiss Systems Overview Axio Scope.A1 22 M60-2-0007 e 05/08 2.2 Systems Overview

TECHNICAL MANUAL Axio Scope.A1 Systems Overview Carl Zeiss M60-2-0007 e 05/08 23

TECHNICAL MANUAL Carl Zeiss Systems Overview Axio Scope.A1 24 M60-2-0007 e 05/08

TECHNICAL MANUAL Axio Scope.A1 Systems Overview Carl Zeiss M60-2-0007 e 05/08 25

TECHNICAL MANUAL Carl Zeiss Systems Overview Axio Scope.A1 26 M60-2-0007 e 05/08

TECHNICAL MANUAL Axio Scope.A1 Technical Data Carl Zeiss M60-2-0007 e 05/08 27 2.3 Technical Data Dimensions (width x depth x height) Micr

TECHNICAL MANUAL Carl Zeiss Technical Data Axio Scope.A1 28 M60-2-0007 e 05/08 Transformer HBO 100 W Range of application...

STARTUP Axio Scope.A1 Mounting the Standard Components Carl Zeiss M60-2-0007 e 05/08 29 3 STARTUP The customer has the choice to either se

Axio Scope.A1 Content / Figures Carl Zeiss M60-2-0007 e 05/08 3 CONTENT Page 1 INTRODUCTION ...

STARTUP Carl Zeiss Mounting the Standard Components Axio Scope.A1 30 M60-2-0007 e 05/08 3.1.2 Mounting the Upper Stand Part on the Stand C

STARTUP Axio Scope.A1 Mounting the Standard Components Carl Zeiss M60-2-0007 e 05/08 31 3.1.3 Mounting the Binocular Tube / Photo Tube All

STARTUP Carl Zeiss Mounting the Standard Components Axio Scope.A1 32 M60-2-0007 e 05/08 How to insert eyepiece graticules The eyepieces PL

STARTUP Axio Scope.A1 Mounting the Standard Components Carl Zeiss M60-2-0007 e 05/08 33 3.1.5 Mounting Objectives • Lower the mechanical

STARTUP Carl Zeiss Mounting the Standard Components Axio Scope.A1 34 M60-2-0007 e 05/08 3.1.6 Installing and Uninstalling "Push&C

STARTUP Axio Scope.A1 Mounting the Standard Components Carl Zeiss M60-2-0007 e 05/08 35 3.1.7 Mounting the Reflector Insert • First remov

STARTUP Carl Zeiss Mounting the Standard Components Axio Scope.A1 36 M60-2-0007 e 05/08 3.1.8 Mounting the Mechanical Stage Depending on

STARTUP Axio Scope.A1 Mounting the Standard Components Carl Zeiss M60-2-0007 e 05/08 37 b) How to mount the stage • Loosen the screw caps

STARTUP Carl Zeiss Mounting the Standard Components Axio Scope.A1 38 M60-2-0007 e 05/08 (4) Removing and mounting the additional sleeves Bo

STARTUP Axio Scope.A1 Mounting the Standard Components Carl Zeiss M60-2-0007 e 05/08 39 3.1.9 Mechanical Stages with Friction Adjustment

Carl Zeiss Content / Figures Axio Scope.A1 4 M60-2-0007 e 05/08 3.3 Switching the Microscope and its Separate Illumination Fixtures on a

STARTUP Carl Zeiss Mounting the Standard Components Axio Scope.A1 40 M60-2-0007 e 05/08 (3) Adjusting the connecting rod position of the e

STARTUP Axio Scope.A1 Mounting the Standard Components Carl Zeiss M60-2-0007 e 05/08 41 3.1.10 Mounting the LED Illuminator for Transmitte

STARTUP Carl Zeiss Mounting the Standard Components Axio Scope.A1 42 M60-2-0007 e 05/08 Guidelines for the use of the LED illuminator acce

STARTUP Axio Scope.A1 Mounting the Standard Components Carl Zeiss M60-2-0007 e 05/08 43 3.1.12 Mounting the Condenser • Slide the stage c

STARTUP Carl Zeiss Mounting the Standard Components Axio Scope.A1 44 M60-2-0007 e 05/08 3.1.13 Mounting the Stage Carrier • Screw the sho

STARTUP Axio Scope.A1 Mounting the Standard Components Carl Zeiss M60-2-0007 e 05/08 45 3.1.14 Inserting the Halogen Lamp 12 V, 50 W •

STARTUP Carl Zeiss Mounting the Standard Components Axio Scope.A1 46 M60-2-0007 e 05/08 3.1.15 Halogen Lamp HAL 100 The halogen lamp HAL 1

STARTUP Axio Scope.A1 Mounting the Standard Components Carl Zeiss M60-2-0007 e 05/08 47 3.1.15.2 Adjusting the halogen lamp HAL 100 Rough

STARTUP Carl Zeiss Mounting the Standard Components Axio Scope.A1 48 M60-2-0007 e 05/08 3.1.15.3 Changing the halogen lamp 12 V, 100 W CA

STARTUP Axio Scope.A1 Mounting the Standard Components Carl Zeiss M60-2-0007 e 05/08 49 3.1.16 Inserting the Adjustment Aid into the Upper

Axio Scope.A1 Content / Figures Carl Zeiss M60-2-0007 e 05/08 5 4.2.9 Upper Stand Part HAL 100/HBO DIC ...

STARTUP Carl Zeiss Mounting the Standard Components Axio Scope.A1 50 M60-2-0007 e 05/08 • Press the spring lever (454H454HFig. 3-28/5) on

STARTUP Axio Scope.A1 Mounting the Standard Components Carl Zeiss M60-2-0007 e 05/08 51 3.1.17.2 Mounting HBO 50 illuminator • Mount HBO

STARTUP Carl Zeiss Mounting the Standard Components Axio Scope.A1 52 M60-2-0007 e 05/08 3.1.18 HBO 100 Illuminator 3.1.18.1 Inserting the

STARTUP Axio Scope.A1 Mounting the Standard Components Carl Zeiss M60-2-0007 e 05/08 53 3.1.18.3 Adjusting the HBO 100 illuminator The HB

STARTUP Carl Zeiss Connection of the Power Supply Axio Scope.A1 54 M60-2-0007 e 05/08 3.2 Connection of the Power Supply 3.2.1 Lower Stan

STARTUP Axio Scope.A1 Switching the Microscope and its Separate Illumination Fixtures on and off Carl Zeiss M60-2-0007 e 05/08 55 3.2.3 Up

STARTUP Carl Zeiss Mounting Optional Components Axio Scope.A1 56 M60-2-0007 e 05/08 3.4 Mounting Optional Components Make sure to unplu

STARTUP Axio Scope.A1 Mounting Optional Components Carl Zeiss M60-2-0007 e 05/08 57 3.4.2 Mounting the Intermediate Plate for Analyzer Sli

STARTUP Carl Zeiss Mounting Optional Components Axio Scope.A1 58 M60-2-0007 e 05/08 3.4.4 Mounting the Magnification Changer • If necessa

STARTUP Axio Scope.A1 Mounting Optional Components Carl Zeiss M60-2-0007 e 05/08 59 When mounting the appropriate emission filter into the

Carl Zeiss Content / Figures Axio Scope.A1 6 M60-2-0007 e 05/08 FIGURES 0Fig. 1-1 Caution sign on lower stand part for HAL 100- and HAL

STARTUP Carl Zeiss Mounting Optional Components Axio Scope.A1 60 M60-2-0007 e 05/08 3.4.6 Changing the Color Splitter in the Reflector Mod

STARTUP Axio Scope.A1 Mounting Optional Components Carl Zeiss M60-2-0007 e 05/08 61 The reflecting (coated) side (550H550HFig. 3-46/3) of

STARTUP Carl Zeiss Mounting Optional Components Axio Scope.A1 62 M60-2-0007 e 05/08 3.4.8 Mounting and Centering the Overview Fixture • I

STARTUP Axio Scope.A1 Mounting Optional Components Carl Zeiss M60-2-0007 e 05/08 63 3.4.9 Changing the LED Module in the Upper Stand Part

STARTUP Carl Zeiss Mounting Optional Components Axio Scope.A1 64 M60-2-0007 e 05/08 3.4.10 Inserting a Modulator Disk in the Condenser 0.9

STARTUP Axio Scope.A1 Mounting Optional Components Carl Zeiss M60-2-0007 e 05/08 65 3.4.11 Changing the Diaphragm PlasDIC How to mount the

STARTUP Carl Zeiss Mounting Optional Components Axio Scope.A1 66 M60-2-0007 e 05/08 3.4.13 Changing the Filter in the Filter Wheel Transmi

STARTUP Axio Scope.A1 Default Setting of the Microscope Carl Zeiss M60-2-0007 e 05/08 67 3.5 Default Setting of the Microscope 3.5.1 Adju

STARTUP Carl Zeiss Default Setting of the Microscope Axio Scope.A1 68 M60-2-0007 e 05/08 3.5.3 Correction of Vision Defects with the Eyepi

OPERATION Axio Scope.A1 Illumination and Contrasting Method Carl Zeiss M60-2-0007 e 05/08 69 4 OPERATION 4.1 Illumination and Contrasting

Axio Scope.A1 Content / Figures Carl Zeiss M60-2-0007 e 05/08 7 44H44HFig. 3-41 Mounting the magnification changer ...

OPERATION Carl Zeiss Illumination and Contrasting Method Axio Scope.A1 70 M60-2-0007 e 05/08 • Center the field diaphragm image with both

OPERATION Axio Scope.A1 Illumination and Contrasting Method Carl Zeiss M60-2-0007 e 05/08 71 (4) Adjusting the vertical stop on the condens

OPERATION Carl Zeiss Illumination and Contrasting Method Axio Scope.A1 72 M60-2-0007 e 05/08 4.1.2 Adjusting the Transmitted Light/Dark-Fi

OPERATION Axio Scope.A1 Illumination and Contrasting Method Carl Zeiss M60-2-0007 e 05/08 73 • Replace the eyepiece into the tube. • Ligh

OPERATION Carl Zeiss Illumination and Contrasting Method Axio Scope.A1 74 M60-2-0007 e 05/08 4.1.3 Adjusting the Transmitted Light/Phase-C

OPERATION Axio Scope.A1 Illumination and Contrasting Method Carl Zeiss M60-2-0007 e 05/08 75 • If the overlap is not exact (632H632HFig. 4

OPERATION Carl Zeiss Illumination and Contrasting Method Axio Scope.A1 76 M60-2-0007 e 05/08 4.1.4 Adjusting the Transmitted Light/Differe

OPERATION Axio Scope.A1 Illumination and Contrasting Method Carl Zeiss M60-2-0007 e 05/08 77 1 DIC slider 2 Knurled screw 3 Objecti

OPERATION Carl Zeiss Illumination and Contrasting Method Axio Scope.A1 78 M60-2-0007 e 05/08 4.1.5 Adjusting PlasDIC-Contrast in Transmitt

OPERATION Axio Scope.A1 Illumination and Contrasting Method Carl Zeiss M60-2-0007 e 05/08 79 4.1.6 Adjusting Transmitted Light/Polarizatio

Carl Zeiss Content / Figures Axio Scope.A1 8 M60-2-0007 e 05/08 90H90HFig. 4-32 Binocular photo tube ...

OPERATION Carl Zeiss Illumination and Contrasting Method Axio Scope.A1 80 M60-2-0007 e 05/08 • Bring the object to be examined into the fi

OPERATION Axio Scope.A1 Illumination and Contrasting Method Carl Zeiss M60-2-0007 e 05/08 81 (3) Adjusting the microscope • Adjust the mi

OPERATION Carl Zeiss Illumination and Contrasting Method Axio Scope.A1 82 M60-2-0007 e 05/08 The phase difference results from the interfer

OPERATION Axio Scope.A1 Illumination and Contrasting Method Carl Zeiss M60-2-0007 e 05/08 83 4.1.7 Adjusting the Reflected Light/Bright-Fi

OPERATION Carl Zeiss Illumination and Contrasting Method Axio Scope.A1 84 M60-2-0007 e 05/08 • Adjust the knurled knob on the field diaphr

OPERATION Axio Scope.A1 Illumination and Contrasting Method Carl Zeiss M60-2-0007 e 05/08 85 1 Centering screw of the aperture diaphrag

OPERATION Carl Zeiss Illumination and Contrasting Method Axio Scope.A1 86 M60-2-0007 e 05/08 4.1.8 Adjusting the Reflected Light/Dark-Fiel

OPERATION Axio Scope.A1 Illumination and Contrasting Method Carl Zeiss M60-2-0007 e 05/08 87 4.1.9 Adjusting Reflected Light DIC and Refle

OPERATION Carl Zeiss Illumination and Contrasting Method Axio Scope.A1 88 M60-2-0007 e 05/08 4.1.10 Adjusting Reflected Light TIC (1) Appl

OPERATION Axio Scope.A1 Illumination and Contrasting Method Carl Zeiss M60-2-0007 e 05/08 89 The values a and b (see 684H684HFig. 4-14) are

INTRODUCTION Axio Scope.A1 Safety Guidelines Carl Zeiss M60-2-0007 e 05/08 9 1 INTRODUCTION 1.1 Safety Guidelines The microscopes Axio Sc

OPERATION Carl Zeiss Illumination and Contrasting Method Axio Scope.A1 90 M60-2-0007 e 05/08 Attention: • If the step and its surrounding

OPERATION Axio Scope.A1 Illumination and Contrasting Method Carl Zeiss M60-2-0007 e 05/08 91 4.1.11 Adjusting Reflected Light Polarization

OPERATION Carl Zeiss Illumination and Contrasting Method Axio Scope.A1 92 M60-2-0007 e 05/08 A sample is bireflective when the sample detai

OPERATION Axio Scope.A1 Illumination and Contrasting Method Carl Zeiss M60-2-0007 e 05/08 93 4.1.12 Adjusting Reflected Light Fluorescence

OPERATION Carl Zeiss Illumination and Contrasting Method Axio Scope.A1 94 M60-2-0007 e 05/08 • Switch on the lamp HBO 100 or HBO 50 (691H6

OPERATION Axio Scope.A1 Operating and Functional Elements of Optional Components Carl Zeiss M60-2-0007 e 05/08 95 4.2 Operating and Functi

OPERATION Carl Zeiss Operating and Functional Elements of Optional Components Axio Scope.A1 96 M60-2-0007 e 05/08 4.2.2 Lower Stand Part w

OPERATION Axio Scope.A1 Operating and Functional Elements of Optional Components Carl Zeiss M60-2-0007 e 05/08 97 4.2.3 Lower Stand Part f

OPERATION Carl Zeiss Operating and Functional Elements of Optional Components Axio Scope.A1 98 M60-2-0007 e 05/08 4.2.4 Stand Column Vario

OPERATION Axio Scope.A1 Operating and Functional Elements of Optional Components Carl Zeiss M60-2-0007 e 05/08 99 4.2.5 Upper Stand Part T