
The zoom and scan field rotation can be set in the Zoom, Rotation, & Offset panel (Offset here refers
to fine XY movement of the scan area). You may find it more convenient to use the crop function in the
"Image" window -
Crop Your Image (optional)
This function is a true optical zoom performed upon image acquisition - the laser will scan a user-
defined region of interest and display the scan data in the designated frame size (512x512, etc). It is a
convenient way of setting the zoom, rotation and offset displayed under the Mode tab.
Press "Crop" from the Image window display toolbar. A crop box appears in the current image window,
which can be moved, rotated and resized with the mouse.
Pressing "single" will rescan with the modified zoom etc settings
(Pressing the "reset" button in the mode panel is useful for undoing all these changes, ready for the
next sample.)
Images are overwritten so save as you
go along
Images are saved as .lsm files within a .mdb
database. Images can be exported as TIFFs
Click the "Save" icon on the bottom right of the image
window
If you don't have a database already, you need to
create one
Make sure you are saving images to the correct
database
Name the image and press save
Users should generally save their images either to their
own user folder on the \\Bio-lemming\Confocal fileserver or
directly to a USB memory drive. Any images saved on the
hard-drives are unsafe and are subject to deletion
WITHOUT WARNING. The Imaging Suite anteroom (4226
FFSC) has a PC workstation with both a USB port and a
DVD writer that can be used for transferring data off the
network to portable storage devices.
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