Zeiss N HBO 103 Spezifikationen Seite 1

Operating Manual Axiovert 200 / Axiovert 200 M Inverted Microscopes

INTRODUCTION Carl Zeiss Notes on warranty Axiovert 200 0-10 B 40-080 e 10/03 Notes on warranty The manufacturer guarantees that the inst

OPERATION Carl Zeiss Illumination and contrasting techniques Axiovert 200 3-40 B 40-080 e 10/03 3.3.5 Setting of fluorescence contrast

OPERATION Axiovert 200 Illumination and contrasting techniques Carl Zeiss B 40-080 e 10/03 3-41 1 N HBO 103 fluorescence illuminato

OPERATION Carl Zeiss Illumination and contrasting techniques Axiovert 200 3-42 B 40-080 e 10/03 • Switch on the NHBO 103 fluorescence i

OPERATION Axiovert 200 Documentation Carl Zeiss B 40-080 e 10/03 3-43 3.4 Documentation Depending on the instrument configuration, the A

OPERATION Carl Zeiss Documentation Axiovert 200 3-44 B 40-080 e 10/03 Order No. / Viewed with / Intermediate / Direction of Viewing

OPERATION Axiovert 200 Documentation Carl Zeiss B 40-080 e 10/03 3-45 Order No. / Viewed with / Intermediate / Direction of Viewing

OPERATION Carl Zeiss Documentation Axiovert 200 3-46 B 40-080 e 10/03 • Attach the premounted camera system to the Frontport, align it

CARE, MAINTENANCE, TROUBLESHOOTING AND SERVICE Axiovert 200 Contents / List of illustrations Carl Zeiss B 40-080 e 10/03 4-1 CARE, MA

CARE, MAINTENANCE, TROUBLESHOOTING AND SERVICE Carl Zeiss Contents / List of illustrations Axiovert 200 4-2 B 40-080 e 10/03

CARE, MAINTENANCE, TROUBLESHOOTING AND SERVICE Axiovert 200 Care Carl Zeiss B 40-080 e 10/03 4-3 4 CARE, MAINTENANCE, TROUBLESHOOTING A

INTRODUCTION Axiovert 200 Overall view of the Axiovert 200 and Axiovert 200 M Carl Zeiss B 40-080 e 10/03 0-11

CARE, MAINTENANCE, TROUBLESHOOTING AND SERVICE Carl Zeiss Maintenance Axiovert 200 4-4 B 40-080 e 10/03 4.2 Maintenance 4.2.1 Perfor

CARE, MAINTENANCE, TROUBLESHOOTING AND SERVICE Axiovert 200 Maintenance Carl Zeiss B 40-080 e 10/03 4-5 4.2.3 Changing the fuses on the

CARE, MAINTENANCE, TROUBLESHOOTING AND SERVICE Carl Zeiss Service Axiovert 200 4-6 B 40-080 e 10/03 4.3 Service All repairs of optical

ANNEX Axiovert 200 Overview Carl Zeiss B 40-080 e 10/03 A-1 Annex: List of abbreviations...

ANNEX Carl Zeiss Overview Axiovert 200 A-2 B 40-080 e 10/03

ANNEX Axiovert 200 List of abbreviations Carl Zeiss B 40-080 e 10/03 A-3 List of abbreviations a Free working distance AC Al

ANNES Carl Zeiss List of abbreviations Axiovert 200 A-4 B 40-080 e 10/03 Ph phase contrast PL flatfield SB Sub-Bus SLR Single Len

ANNEX Axiovert 200 List of key words Carl Zeiss B 40-080 e 10/03 A-5 List of key words Page 3 3.200K ...

ANNEX Carl Zeiss List of key words Axiovert 200 A-6 B 40-080 e 10/03 F fluorescence contrast...

ANNEX Axiovert 200 List of key words Carl Zeiss B 40-080 e 10/03 A-7 P parfocality ...

INTRODUCTION Carl Zeiss Microscopy in transmitted-light brightfield in a few steps Axiovert 200 0-12 B 40-080 e 10/03 Microscopy in tran

ANNEX Carl Zeiss Patent rights Axiovert 200 A-8 B 40-080 e 10/03 Patent rights The instruments, instrument components or methods describe

INTRODUCTION Axiovert 200 Microscopy in transmitted-light brightfield in a few steps Carl Zeiss B 40-080 e 10/03 0-13 1 On / Off swi

INTRODUCTION Carl Zeiss Microscopy in transmitted-light brightfield in a few steps Axiovert 200 0-14 B 40-080 e 10/03

INSTRUMENT DESCRIPTION Axiovert 200 Contents / List of illustrations Carl Zeiss B 40-080 e 10/03 1-1 INSTRUMENT DESCRIPTION Contents

INSTRUMENT DESCRIPTION Carl Zeiss Contents / List of illustrations Axiovert 200 1-2 B 40-080 e 10/03

INSTRUMENT DESCRIPTION Axiovert 200 Name and intended application Carl Zeiss B 40-080 e 10/03 1-3 1 INSTRUMENT DESCRIPTION 1.1 Name and

INSTRUMENT DESCRIPTION Carl Zeiss Name and intended application Axiovert 200 1-4 B 40-080 e 10/03 Typical fields of application: Examina

INSTRUMENT DESCRIPTION Axiovert 200 Instrument description and main features Carl Zeiss B 40-080 e 10/03 1-5 1.2 Instrument description

INTRODUCTION Carl Zeiss Copyright Axiovert 200 0-2 B 40-080 e 10/03 Knowledge of this manual is required for the operation of the instru

INSTRUMENT DESCRIPTION Carl Zeiss Microscope configurations and modules Axiovert 200 1-6 B 40-080 e 10/03 1.3 Microscope configurations

INSTRUMENT DESCRIPTION Axiovert 200 Microscope configurations and modules Carl Zeiss B 40-080 e 10/03 1-7 Fig. 1-2 Microscope configu

INSTRUMENT DESCRIPTION Carl Zeiss Microscope configurations and modules Axiovert 200 1-8 B 40-080 e 10/03 Fig. 1-3 Microscope configu

INSTRUMENT DESCRIPTION Axiovert 200 Microscope configurations and modules Carl Zeiss B 40-080 e 10/03 1-9 Fig. 1-4 Microscope configu

INSTRUMENT DESCRIPTION Carl Zeiss Objectives Axiovert 200 1-10 B 40-080 e 10/03 1.4 Objectives The objectives are the optical centerpiec

INSTRUMENT DESCRIPTION Axiovert 200 Objectives Carl Zeiss B 40-080 e 10/03 1-11 The following objectives are available for the Axiovert

INSTRUMENT DESCRIPTION Carl Zeiss Eyepieces / Condensers Axiovert 200 1-12 B 40-080 e 10/03 1.5 Eyepieces The following eyepieces are av

INSTRUMENT DESCRIPTION Axiovert 200 Specimen stages and mounting frames / Binocular tubes Carl Zeiss B 40-080 e 10/03 1-13 1.7 Specimen

INSTRUMENT DESCRIPTION Carl Zeiss Technical Data Axiovert 200 1-14 B 40-080 e 10/03 1.9 Technical Data Dimensions (width x depth x heigh

INSTRUMENT DESCRIPTION Axiovert 200 Technical Data Carl Zeiss B 40-080 e 10/03 1-15 Line frequency ...

INTRODUCTION Axiovert 200 Contents Carl Zeiss B 40-080 e 10/03 0-3 CONTENTS page INTRODUCTION...

INSTRUMENT DESCRIPTION Carl Zeiss Technical Data Axiovert 200 1-16 B 40-080 e 10/03

START-UP Axiovert 200 Contents Carl Zeiss B 40-080 e 10/03 2-1 START-UP Contents 2 START-UP ...

START-UP Carl Zeiss Contents Axiovert 200 2-2 B 40-080 e 10/03 2.8 Connection to the line ...

START-UP Axiovert 200 List of illustrations Carl Zeiss B 40-080 e 10/03 2-3 List of illustrations Fig. 2-1 Installation of microscope..

START-UP Carl Zeiss List of illustrations Axiovert 200 2-4 B 40-080 e 10/03

START-UP Axiovert 200 Unpacking and installation of microscope Carl Zeiss B 40-080 e 10/03 2-5 2 START-UP On account of the complexity

START-UP Carl Zeiss Attachment of binocular (photo)tube Axiovert 200 2-6 B 40-080 e 10/03 2.2 Attachment of binocular (photo) tube All

START-UP Axiovert 200 Attachment of binocular (photo)tube Carl Zeiss B 40-080 e 10/03 2-7 2.2.2 Inserting the eyepiece reticle The eyep

START-UP Carl Zeiss Attachment of transmitted-light illumination Axiovert 200 2-8 B 40-080 e 10/03 2.3 Attachment of transmitted-light

START-UP Axiovert 200 Screw in objectives Carl Zeiss B 40-080 e 10/03 2-9 2.4 Screw in objectives • Remove dust caps (2-7/1) from the

INTRODUCTION Carl Zeiss Contents Axiovert 200 0-4 B 40-080 e 10/03 page 2.4 Screw in objectives ...

START-UP Carl Zeiss Attachment of microscope stages Axiovert 200 2-10 B 40-080 e 10/03 2.5 Attachment of microscope stages 2.5.1 Attac

START-UP Axiovert 200 Attachment of microscope stages Carl Zeiss B 40-080 e 10/03 2-11 Because of the large travel range of the scannin

START-UP Carl Zeiss Attachment of microscope stages Axiovert 200 2-12 B 40-080 e 10/03 2.5.4 Attachment of heating stage The heating st

START-UP Axiovert 200 Attachment of condensers Carl Zeiss B 40-080 e 10/03 2-13 2.6 Attachment of condensers 2.6.1 Condensers for the

START-UP Carl Zeiss Attachment of condensers Axiovert 200 2-14 B 40-080 e 10/03 • Fix condenser adapter in position with clamping screw

START-UP Axiovert 200 Reflector turret Carl Zeiss B 40-080 e 10/03 2-15 2.7 Reflector turret 2.7.1 Attachment of reflector turret The m

START-UP Carl Zeiss Reflector turret Axiovert 200 2-16 B 40-080 e 10/03 • To remove a reflector module no longer required, first pull i

START-UP Axiovert 200 Reflector turret Carl Zeiss B 40-080 e 10/03 2-17 • Hold both halves of the reflector module together, turn them

START-UP Carl Zeiss Connection to the line Axiovert 200 2-18 B 40-080 e 10/03 2.8 Connection to the line • Connect microscope socket (

START-UP Axiovert 200 Interfaces ... / Microscope and power supply ... Carl Zeiss B 40-080 e 10/03 2-19 2.9 Interfaces of the Axiovert

INTRODUCTION Axiovert 200 Contents Carl Zeiss B 40-080 e 10/03 0-5 page CHAPTER 3 OPERATION ...

START-UP Carl Zeiss HAL 100 W halogen illuminator Axiovert 200 2-20 B 40-080 e 10/03 2.12 HAL 100 halogen illuminator 2.12.1 Change /

START-UP Axiovert 200 HAL 100 W halogen illuminator Carl Zeiss B 40-080 e 10/03 2-21 2.12.2 Coarse alignment of halogen illuminator •

START-UP Carl Zeiss HAL 100 W halogen illuminator Axiovert 200 2-22 B 40-080 e 10/03 2.12.4 Fine alignment of halogen illuminator Fine

START-UP Axiovert 200 Illuminator N HBO 103 Carl Zeiss B 40-080 e 10/03 2-23 2.13 Illuminator N HBO 103 2.13.1 Attachment / change of

START-UP Carl Zeiss Illuminator N HBO 103 Axiovert 200 2-24 B 40-080 e 10/03 • Use focusing knob (2-30/3) to bring collector to the pos

START-UP Axiovert 200 Illuminator N HBO 103 Carl Zeiss B 40-080 e 10/03 2-25 HBO 103 The following settings for adjustment of the N HBO

START-UP Carl Zeiss Illuminator N HBO 103 Axiovert 200 2-26 B 40-080 e 10/03 2.13.3 Attachment of illuminator N HBO 103 • Remove cove

START-UP Axiovert 200 Illuminator N HBO 103 Carl Zeiss B 40-080 e 10/03 2-27 2.13.4 Fine adjustment of illuminator N HBO 103 using the

START-UP Carl Zeiss Attachment of Aqua Stop Axiovert 200 2-28 B 40-080 e 10/03 2.14 Attachment of Aqua Stop Aqua Stop can be used to pr

START-UP Axiovert 200 Attachment of Aqua Stop Carl Zeiss B 40-080 e 10/03 2-29 • You can fix the drain hose using the provided clips. ☞

INTRODUCTION Carl Zeiss Notes on instrument safety Axiovert 200 0-6 B 40-080 e 10/03 Notes on instrument safety The Axiovert 200 / Axiov

START-UP Carl Zeiss Attachment of Aqua Stop Axiovert 200 2-30 B 40-080 e 10/03 Objective type Magnification / num. aperture Contrasting

OPERATION Axiovert 200 Contents Carl Zeiss B 40-080 e 10/03 3-1 OPERATION Contents 3 OPERATION...

OPERATION Carl Zeiss List of illustrations Axiovert 200 3-2 B 40-080 e 10/03 List of illustrations Fig. 3-1 Operation and function cont

OPERATION Axiovert 200 Carl Zeiss B 40-080 e 10/03 3-3 3 OPERATION The Axiovert 200 microscopes are offered in a manual version and in

OPERATION Carl Zeiss Axiovert 200 Axiovert 200 3-4 B 40-080 e 10/03 3.1 Axiovert 200 (manual) 3.1.1 Operation and function controls on

OPERATION Axiovert 200 Axiovert 200 Carl Zeiss B 40-080 e 10/03 3-5 Key to Fig. 3-1: 1 On / Off switch 2 Nosepiece 3 Compartmen

OPERATION Carl Zeiss Axiovert 200 Axiovert 200 3-6 B 40-080 e 10/03 On / Off switch (3-1/1) − position 0 = instrument switched off − p

OPERATION Axiovert 200 Axiovert 200 Carl Zeiss B 40-080 e 10/03 3-7 Nosepiece with objectives (3-1/2) − 6-position nosepiece H, DIC (3-

OPERATION Carl Zeiss Axiovert 200 Axiovert 200 3-8 B 40-080 e 10/03 Binocular tubes (3-1/13) − The binocular tubes offered permit the i

OPERATION Axiovert 200 Axiovert 200 Carl Zeiss B 40-080 e 10/03 3-9 Binocular component of the tubes (3-1/14) − The eyepiece distance

INTRODUCTION Axiovert 200 Notes on instrument safety Carl Zeiss B 40-080 e 10/03 0-7 The Axiovert 200 microscopes, including original acc

OPERATION Carl Zeiss Axiovert 200 Axiovert 200 3-10 B 40-080 e 10/03 Condensers (3-1/23) Depending on their type, condensers (3-7/1) ar

OPERATION Axiovert 200 Axiovert 200 Carl Zeiss B 40-080 e 10/03 3-11 Centering screw for condenser (3-1/18 and 22) − Screws for conden

OPERATION Carl Zeiss Axiovert 200 Axiovert 200 3-12 B 40-080 e 10/03 Baseport (3-1/34) − Port for the connection of documentation equ

OPERATION Axiovert 200 Axiovert 200 Carl Zeiss B 40-080 e 10/03 3-13 5-position reflector turret (3-1/7) − Accepts the maximum of 5 re

OPERATION Carl Zeiss Axiovert 200 Axiovert 200 3-14 B 40-080 e 10/03 3.1.2 Switching on and basic settings on the Axiovert 200 (manual)

OPERATION Axiovert 200 Axiovert 200 Carl Zeiss B 40-080 e 10/03 3-15 3.1.2.2 Light Manager The Light Manager is automatically activated

OPERATION Carl Zeiss Axiovert 200 M Axiovert 200 3-16 B 40-080 e 10/03 3.2 Axiovert 200 M (motorized) 3.2.1 Operation and function con

OPERATION Axiovert 200 Axiovert 200 M Carl Zeiss B 40-080 e 10/03 3-17 Key to Fig. 3-13: 1 On / Off switch 2 Nosepiece (motorized)

OPERATION Carl Zeiss Axiovert 200 M Axiovert 200 3-18 B 40-080 e 10/03 FOCUS key - Work / Load (3-13/8) − Load key Brings the nosepi

OPERATION Axiovert 200 Axiovert 200 M Carl Zeiss B 40-080 e 10/03 3-19 LCD display (3-13/17) − Two-line display with 16 characters ea

INTRODUCTION Carl Zeiss Notes on instrument safety Axiovert 200 0-8 B 40-080 e 10/03 Gas discharge lamps, e.g. HBO 100, emit ultraviole

OPERATION Carl Zeiss Axiovert 200 M Axiovert 200 3-20 B 40-080 e 10/03 Motorized condensers (3-13/16) Motorized condenser, equipped with

OPERATION Axiovert 200 Axiovert 200 M Carl Zeiss B 40-080 e 10/03 3-21 LEFT / RIGHT key for the motorized switching of the Sideport turr

OPERATION Carl Zeiss Axiovert 200 M Axiovert 200 3-22 B 40-080 e 10/03 Motorized 5-position reflector turret (3-13/3) − Accepts the ma

OPERATION Axiovert 200 Axiovert 200 M Carl Zeiss B 40-080 e 10/03 3-23 3.2.2 Switching on and basic settings on the Axiovert 200 M (mot

OPERATION Carl Zeiss Axiovert 200 M Axiovert 200 3-24 B 40-080 e 10/03 Setting of nosepiece equipment • Press one of the two OBJECTIVE

OPERATION Axiovert 200 Axiovert 200 M Carl Zeiss B 40-080 e 10/03 3-25 Setting of Z focus factors and parfocality The matching of focus

OPERATION Carl Zeiss Axiovert 200 M Axiovert 200 3-26 B 40-080 e 10/03 Deactivation of Set mode and saving of settings The Set mode can

OPERATION Axiovert 200 Axiovert 200 M Carl Zeiss B 40-080 e 10/03 3-27 3.2.2.3 Light Manager The Light Manager is applied with the Axiov

OPERATION Carl Zeiss Illumination and contrasting techniques Axiovert 200 3-28 B 40-080 e 10/03 3.3 Illumination and contrasting techni

OPERATION Axiovert 200 Illumination and contrasting techniques Carl Zeiss B 40-080 e 10/03 3-29 3.3.1.3 Transmitted-light brightfield s

INTRODUCTION Axiovert 200 Notes on instrument safety Carl Zeiss B 40-080 e 10/03 0-9 ☞ Be sure to read the safety notes provided with Imm

OPERATION Carl Zeiss Illumination and contrasting techniques Axiovert 200 3-30 B 40-080 e 10/03 1 On / Off switch 2 Nosepiece 3

OPERATION Axiovert 200 Illumination and contrasting techniques Carl Zeiss B 40-080 e 10/03 3-31 • Swing out 3-position filter changer (

OPERATION Carl Zeiss Illumination and contrasting techniques Axiovert 200 3-32 B 40-080 e 10/03 3.3.1.4 Transmitted-light brightfield c

OPERATION Axiovert 200 Illumination and contrasting techniques Carl Zeiss B 40-080 e 10/03 3-33 3.3.2 Setting of transmitted-light phas

OPERATION Carl Zeiss Illumination and contrasting techniques Axiovert 200 3-34 B 40-080 e 10/03 3.3.2.3 Setting of transmitted-light ph

OPERATION Axiovert 200 Illumination and contrasting techniques Carl Zeiss B 40-080 e 10/03 3-35 3.3.3 Setting of differential interfere

OPERATION Carl Zeiss Illumination and contrasting techniques Axiovert 200 3-36 B 40-080 e 10/03 (2) Transmitted-light DIC with analyzer

OPERATION Axiovert 200 Illumination and contrasting techniques Carl Zeiss B 40-080 e 10/03 3-37 1 Polarizer D (fixed, optional: rot

OPERATION Carl Zeiss Illumination and contrasting techniques Axiovert 200 3-38 B 40-080 e 10/03 3.3.4 Setting of VAREL contrast in tran

OPERATION Axiovert 200 Illumination and contrasting techniques Carl Zeiss B 40-080 e 10/03 3-39 The specimen field with the relevant pup