Zeiss AxioCam MRc5 Bedienungsanleitung PDF herunterladen (Seite 4)

Min/Max is: optimum of the minimum and maximum contained gray or color value.

Linear (Max. range) is: linear display of the full "dynamic" range of possible values.

Gamma 0.45 is: preset display curve for best color reproduction. Below, Linear is selected (light

blue highlight), but use whichever gives the truest or most desired image; Min/Max can be a

good start because it scales specifically over the particular pixel intensity range of the image.

The active image histogram display for each of these functions can be viewed by selecting

Display tab in the menu (to the left of Adjust tab). These scaling functions also can be selected

for by clicking in their respective boxes in Display. The use of features in the Display menu will

be mentioned in the post-image acquisition section, below.

Focus the sample's image on the screen, and choose a desired brightness level. [* note that, the

ocular lenses can be adjusted to give a focus for the user's eyes that matches the focus for the

digital image on the monitor.] remember, image brightness from the camera is determined by

both the lamp intensity setting (halogen) and the camera shutter exposure time.] The halogen

lamp, used for brightfield and DIC imaging, has an intensity adjustment knob which controls the

level of lamp brightness. A good starting point for halogen lamp intensity is usually between 5—

7; the exposure time can be chosen by sliding the bar horizontally with the left mouse click

depressed or entering a number with the keyboard (below, blue arrow). To make small changes

in time values, the left and right arrows can be clicked on. It should not be necessary to have

the lamp intensity at a value greater than 7; over 7 is too "hot." (Longer exposure time settings

tend to be a better way to increase image brightness.) When set at an average brightness or

"temperature" (approximately 4-6), the halogen lamp has a "black-body temperature" of about

3200K (as described below). If the 3200K box in the White Balance display area is used (below,

red arrow), the white balance will be adjusted to a basic setting based upon a light source

temperature of 3200K. This white balance adjustment is related to halogen lamp use (white

transmitted light), and affects the color balance of the sample. Note: There are neutral density

filters at the right base of the microscope; these will affect the color balance of the halogen

lamp. The true temperature of the lamp (3200K) is when these filters are not in place (sticks in

OUT position). If the mercury arc lamp is used (for fluorescence microscopy and imaging), white

balancing using a reference white background in the sample can not be directly performed

because there is no white light. [Light used for fluorophore excitation is of a particular

wavelength range (color); the sample's emitted light will also be of a particular wavelength

range (color).] As stated below, use of Interactive... or Automatic white balancing for

fluorescence can result in color distortion, and should be avoided. With the 3200K off, RGB

color balancing can be done manually using the RGB arrowheads or entering numbers in the

boxes (below, black arrow). If colors from the camera, as seen on the monitor, are still not the

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Zeiss AxioCam MRc5 Bedienungsanleitung Seite 4