Zeiss LSM 510 META Spezifikationen
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- Inhaltsverzeichnis
- LESEZEICHEN
- Laser Scanning Microscope 1
- B 40-051 e 07/98 III 3
- IV B 40-051 e 07/98 4
- Scope of Equipment Supplied 6
- INTRODUCTION 10
- X B 40-051 e 07/98 10
- CONTENTS 11
- NOTES ON DEVICE SAFETY 12
- Contents LSM 510 12
- 1-2 B 40-051 e 07/98 12
- 1 NOTES ON DEVICE SAFETY 13
- Exit aperture 18
- B 40-051 e 07/98 1-9 19
- 1-10 B 40-051 e 07/98 20
- LGK 7774 21
- LGK 7628-1 21
- 1-12 B 40-051 e 07/98 22
- LSM 510 - SETUP REQUIREMENTS 24
- 2-2 B 40-051 e 07/98 24
- Fig. 2-1 25
- Fig. 2-2 25
- Fig. 2-3 26
- Fig. 2-4 27
- 2-12 B 40-051 e 07/98 34
- CHAPTER 3 INTRODUCTION TO 35
- LASER SCANNING MICROSCOPY 35
- 3-2 B 40-051 e 07/98 36
- Module VIS 40
- Scan ModuleMicroscope 40
- Module UV 40
- 3-12 B 40-051 e 07/98 46
- CHAPTER 4 OPERATION 59
- 4 OPERATION 61
- Fig. 4-1 62
- Fig. 4-2 63
- Fig. 4-3 63
- Fig. 4-4 64
- Fig. 4-5 65
- Fig. 4-6 66
- Fig. 4-7 66
- Fig. 4-8 67
- Fig. 4-9 67
- Fig. 4-10 68
- Fig. 4-11 69
- Fig. 4-12 70
- Fig. 4-13 71
- Fig. 4-14 72
- Fig. 4-15 73
- Fig. 4-16 74
- Fig. 4-17 75
- Fig. 4-18 76
- Fig. 4-19 77
- Fig. 4-20 78
- Fig. 4-21 79
- Fig. 4-22 80
- Fig. 4-23 82
- Fig. 4-24 82
- Fig. 4-25 83
- Fig. 4-26 83
- Fig. 4-27 83
- Fig. 4-28 84
- Fig. 4-29 84
- Fig. 4-30 85
- Fig. 4-31 86
- Fig. 4-32 88
- Fig. 4-33 89
- Fig. 4-34 90
- Fig. 4-35 90
- Fig. 4-36 91
- Fig. 4-37 92
- Fig. 4-38 93
- Fig. 4-39 95
- Fig. 4-40 96
- Fig. 4-41 97
- Fig. 4-42 98
- Fig. 4-43 100
- Fig. 4-44 101
- Fig. 4-45 103
- OPERATION 104
- 4-46 B 40-51 e 07/98 104
- B 40-051 e 07/98 4-47 105
- Fig. 4-48 106
- Fig. 4-49 107
- Fig. 4-50 108
- Fig. 4-51 109
- Fig. 4-52 110
- Fig. 4-53 111
- Fig. 4-54 112
- Fig. 4-55 113
- Fig. 4-56 114
- Fig. 4-57 115
- Fig. 4-58 115
- Fig. 4-59 116
- Fig. 4-60 117
- Fig. 4-61 118
- Fig. 4-62 119
- Fig. 4-63 120
- Fig. 4-64 121
- Fig. 4-65 122
- Fig. 4-66 123
- B 40-051 e 07/98 4-67 125
- Fig. 4-68 126
- Colored marker positions 126
- Fig. 4-69 127
- Fig. 4-70 128
- Fig. 4-71 129
- Fig. 4-72 129
- Fig. 4-73 130
- Fig. 4-74 130
- Fig. 4-75 131
- Fig. 4-76 131
- Fig. 4-77 132
- Fig. 4-78 133
- Fig. 4-79 133
- Fig. 4-81 134
- Fig. 4-80 134
- Fig. 4-82 135
- Fig. 4-83 135
- Fig. 4-90 139
- Fig. 4-91 140
- LSM 510 Image Optimization 141
- B 40-051 e 07/98 4-83 141
- Fig. 4-93 142
- Fig. 4-94 143
- Fig. 4-95 144
- B 40-051 e 07/98 4-87 145
- Fig. 4-97 146
- Fig. 4-98 147
- Fig. 4-99 148
- Fig. 4-100 149
- Fig. 4-101 150
- Fig. 4-102 151
- Fig. 4-103 152
- Fig. 4-104 153
- / Ampl. Gain 154
- Fig. 4-105 155
- Fig. 4-106 156
- Fig. 4-107 157
- Fig. 4-108 158
- Fig. 4-109 159
- Fig. 4-110 160
- Fig. 4-111 161
- Fig. 4-112 162
- Fig. 4-113 163
- Fig. 4-114 164
- Fig. 4-115 164
- Fig. 4-116 165
- Fig. 4-117 166
- Fig. 4-118 168
- Fig. 4-119 169
- Fig. 4-120 170
- Fig. 4-121 171
- Fig. 4-122 172
- Fig. 4-123 173
- Fig. 4-124 174
- Fig. 4-125 175
- Fig. 4-126 176
- Fig. 4-127 177
- Fig. 4-128 178
- Fig. 4-129 179
- Fig. 4-130 180
- Fig. 4-131 181
- 4-124 B 40-051 e 07/98 182
- Fig. 4-133 183
- Fig. 4-134 185
- Fig. 4-135 186
- Fig. 4-136 187
- Fig. 4-137 188
- Fig. 4-138 188
- Fig. 4-139 189
- Fig. 4-140 190
- Fig. 4-141 191
- 4-134 B 40-051 e 07/98 192
- Fig. 4-143 193
- Fig. 4-144 194
- B 40-051 e 07/98 4-137 195
- Fig. 4-146 196
- Fig. 4-147 196
- Fig. 4-148 198
- Fig. 4-149 199
- Fig. 4-150 199
- Fig. 4-151 199
- Fig. 4-152 200
- Fig. 4-153 201
- Fig. 4-154 202
- Fig. 4-155 203
- Fig. 4-156 203
- Fig. 4-157 204
- Fig. 4-158 205
- Fig. 4-159 206
- Fig. 4-160 206
- Fig. 4-161 207
- Fig. 4-162 207
- Fig. 4-163 209
- Fig. 4-164 210
- Fig. 4-165 212
- Fig. 4-166 214
- Fig. 4-167 215
- Fig. 4-168 216
- Fig. 4-169 217
- LSM 510 Annex 219
- B 40-051 e 07/98 4-161 219
- Annex LSM 510 220
- 4-162 B 40-051 e 07/98 220
- B 40-051 e 07/98 4-163 221
- 4-164 B 40-051 e 07/98 222
- B 40-051 e 07/98 4-165 223
- 4-166 B 40-051 e 07/98 224
- B 40-051 e 07/98 4-167 225
- 4-168 B 40-051 e 07/98 226
- B 40-051 e 07/98 4-169 227
- 4-170 B 40-051 e 07/98 228
- Fig. 4-170 Filter change 229
- Allen key 230
- B 40-051 e 07/98 4-173 231
- Fine focusing 233
- Fig. 4-173 234
- Fig. 4-174 237
- Fig. 4-175 240
- Fig. 4-176 246
- Fig. 4-177 247
- Fig. 4-178 249
- Fig. 4-179 250
- Fig. 4-180 252
- Fig. 4-181 253
- CHAPTER 5 3D FOR LSM 510 255
- 5-2 B 40-051 e 07/98 256
- Positive rotation directions 257
- Fig. 5-1 257
- Fig. 5-2 259
- Display window 260
- Fig. 5-4 265
- Fig. 5-5 267
- Fig. 5-6 268
- Fig. 5-7 270
- Fig. 5-9 274
- Fig. 5-10 275
- Fig. 5-11 276
- Fig. 5-12 278
- Fig. 5-13 280
- Fig. 5-14 283
- Fig. 5-15 285
- Fig. 5-16 287
- Fig. 5-17 288
- Fig. 5-18 291
- Fig. 5-19 291
- Fig. 5-20 292
- Fig. 5-21 293
- Fig. 5-22 295
- Fig. 5-23 297
- Fig. 5-24 299
- Fig. 5-25 301
- Fig. 5-26 303
- Fig. 5-27 304
- Fig. 5-28 305
- Fig. 5-29 306
- Fig. 5-30 307
- Fig. 5-31 308
- Fig. 5-32 309
- Fig. 5-33 310
- Fig. 5-34 314
- Functions LSM 510 318
- 5-64 B 40-051 e 07/98 318
- Measurement Concept 318
- Fig. 5-36 319
- Fig. 5-37 320
- Fig. 5-39 327
- Measured 329
- Object Features 329
- Volume Features 329
Inhaltsverzeichnis
ILSM 510Laser Scanning MicroscopeOperating Manual
INTRODUCTIONLSM 510X B 40-051 e 07/98
OPERATIONImage Acquisition (Acquire) LSM 5104-42 B 40-51 e 07/984.5.3.2 Tracks functionIf the existing four channels are not sufficient for image ac
OPERATIONLSM 510 Image Acquisition (Acquire)B 40-051 e 07/98 4-43(2) Click on the <Show Tracks> button on the right side of the window.Within
OPERATIONImage Acquisition (Acquire) LSM 5104-44 B 40-51 e 07/98<Add Track> button An additional track is added to the configuration list. The
OPERATIONLSM 510 Image Acquisition (Acquire)B 40-051 e 07/98 4-45<Frame> button The entire area is scanned, and then the parameters are reset.
OPERATIONImage Acquisition (Acquire) LSM 5104-46 B 40-51 e 07/98Clicking the <Scan> button in the LSM 510 tool bar and the <Channels> bu
OPERATIONLSM 510 Image Acquisition (Acquire)B 40-051 e 07/98 4-47The following scan image shows the result with two defined tracks plus the ratio tr
OPERATIONImage Acquisition (Acquire) LSM 5104-48 B 40-51 e 07/98The Ratio Settings box in the "Configuration Control" window makes it poss
OPERATIONLSM 510 Image Acquisition (Acquire)B 40-051 e 07/98 4-49Laser attenuationOn the Beam Path and Channel Assignmentpanel, move the cursor to t
OPERATIONImage Acquisition (Acquire) LSM 5104-50 B 40-51 e 07/98Use the AOTF to set the laser intensity not completely to zero, but only to 0.1 %.Th
OPERATIONLSM 510 Image Acquisition (Acquire)B 40-051 e 07/98 4-51Beam path - Main beam splitterOn the Beam Path and Channel Assignment panel, move t
NOTES ON DEVICE SAFETYLSM 510 ContentsB 40-051 e 07/98 1-1CHAPTER 1 NOTES ON DEVICE SAFETYCONTENTS1 NOTES ON DEVICE SAFETY...
OPERATIONImage Acquisition (Acquire) LSM 5104-52 B 40-51 e 07/98Clicking on the OFF spot results in deactivation of the corresponding channel.Furthe
OPERATIONLSM 510 Image Acquisition (Acquire)B 40-051 e 07/98 4-53Emission filterOn the Beam Path and Channel Assignmentpanel, move the cursor to the
OPERATIONImage Acquisition (Acquire) LSM 5104-54 B 40-51 e 07/984.5.4 Scanning modesTaking a simple configuration as an example, the sections below
OPERATIONLSM 510 Image Acquisition (Acquire)B 40-051 e 07/98 4-554.5.4.1 FrameBefore starting, click on the <Mode> button onthe upper toolbar.
OPERATIONImage Acquisition (Acquire) LSM 5104-56 B 40-51 e 07/98On the Zoom, Rotation & Offset panel, use the slider to set the desired zoom fac
OPERATIONLSM 510 Image Acquisition (Acquire)B 40-051 e 07/98 4-574.5.4.2 Z modeThis mode allows you to create a stack of imagesat different Z levels
OPERATIONImage Acquisition (Acquire) LSM 5104-58 B 40-51 e 07/98The Optical Slice dialog box displays the following information:Black:Stack Z Size (
OPERATIONLSM 510 Image Acquisition (Acquire)B 40-051 e 07/98 4-59Cutline and RangeFor a useful evaluation of the z stack you must proceed as follows
OPERATIONImage Acquisition (Acquire) LSM 5104-60 B 40-51 e 07/98Click on the <Range> button.The image window marks the range to be scanned by
OPERATIONLSM 510 Image Acquisition (Acquire)B 40-051 e 07/98 4-61Mark First/LastOptimum stack size can be obtained by manualfocusing during scan mov
NOTES ON DEVICE SAFETYContents LSM 5101-2 B 40-051 e 07/98
OPERATIONImage Acquisition (Acquire) LSM 5104-62 B 40-51 e 07/98When the <Z Stack> and <Mode> buttons areactivated, objective can be cha
OPERATIONLSM 510 Image Acquisition (Acquire)B 40-051 e 07/98 4-634.5.4.3 ROI function / Region of InterestA scan image allows certain areas (ROIs)
OPERATIONImage Acquisition (Acquire) LSM 5104-64 B 40-51 e 07/98(6) The "Add ROI List" window will appear. Enter any required name to sto
OPERATIONLSM 510 Image Acquisition (Acquire)B 40-051 e 07/98 4-65The following is an example of a scanning procedure where the ROI function was acti
OPERATIONImage Acquisition (Acquire) LSM 5104-66 B 40-51 e 07/984.5.4.4 Time Series functionThe Time Series function offers the following options f
OPERATIONLSM 510 Image Acquisition (Acquire)B 40-051 e 07/98 4-67"Time Series Control" window enabling the entry of parameters for time se
OPERATIONImage Acquisition (Acquire) LSM 5104-68 B 40-51 e 07/98The following example of a scanning image was taken using the Time Series function.
OPERATIONLSM 510 Image Acquisition (Acquire)B 40-051 e 07/98 4-694.5.4.5 Edit Bleach functionThe use of this function permits the intensebleaching
OPERATIONImage Acquisition (Acquire) LSM 5104-70 B 40-51 e 07/98Click on the <Define Region> button.The "Bleach Regions" window appe
OPERATIONLSM 510 Routine ModeB 40-051 e 07/98 4-714.6 Routine ModeThe Routine Mode of the LSM 510 softwarepermits the fast and easy acquisition of
NOTES ON DEVICE SAFETYLSM 510 General / RegulationsB 40-051 e 07/98 1-31 NOTES ON DEVICE SAFETY1.1 GeneralThe LSM 510 laser scanning microscope, in
OPERATIONRoutine Mode LSM 5104-72 B 40-051 e 07/98Select the method in the filed Name and clickon the <Standard> button. An icon appearsbeside
OPERATIONLSM 510 Routine ModeB 40-051 e 07/98 4-734.6.2 Apply Standard ExaminationMethods in the Routine ModeClick on the button of the required sta
OPERATIONRoutine Mode LSM 5104-74 B 40-051 e 07/98Click on <Save> or <Save As> to store theacquired image.Click on <Finish> to exi
OPERATIONLSM 510 Routine ModeB 40-051 e 07/98 4-754.6.3 Export User-Defined Examination Methods to the Routine ModeClick on the <Start Expert Mo
OPERATIONRoutine Mode LSM 5104-76 B 40-051 e 07/98Click on the <Options> button of the main menu and then on <Export To> (Fig. 4-78).The
OPERATIONLSM 510 Routine ModeB 40-051 e 07/98 4-77Click on the <Standard> or the <Mark for List>button.Close the "Select Method To
OPERATIONRoutine Mode LSM 5104-78 B 40-051 e 07/984.6.6 Acquisition of a Z Stack in theRoutine ModeLoad the required stack, the parameters ofwhich
OPERATIONLSM 510 Routine ModeB 40-051 e 07/98 4-79If you are satisfied with the setting, start byclicking on <Next>.The "Routine Mode - I
OPERATIONRoutine Mode LSM 5104-80 B 40-051 e 07/98You can now select Z-stack limits (symmetricallyto the central Z-frame), the number of stepsand th
OPERATIONLSM 510 Image OptimizationB 40-051 e 07/98 4-814.7 Image optimizationSingle framesDescribed below is the example of the acquisition of an i
NOTES ON DEVICE SAFETYNotes on setting up the microscope system LSM 5101-4 B 40-051 e 07/981.3 Notes on setting up the microscope system☞Setting up
OPERATIONImage Optimization LSM 5104-82 B 40-051 e 07/98In the main menu click on the <Scan> button.This opens the "Scan Control" wi
OPERATIONLSM 510 Image OptimizationB 40-051 e 07/98 4-83Click on the <Channels> button.This displays the preset parameters of the configuratio
OPERATIONImage Optimization LSM 5104-84 B 40-051 e 07/98Click on the <Find> button. Make sure to position the slider correctly. Then scan whil
OPERATIONLSM 510 Image OptimizationB 40-051 e 07/98 4-854.7.1 Detector Gain/Ampl. Offset/Ampl. GainIn the "Scan Control" window, click on
OPERATIONImage Optimization LSM 5104-86 B 40-051 e 07/98In the Color Palette List panel, click on the "Range Indicator" item.The scanned i
OPERATIONLSM 510 Image OptimizationB 40-051 e 07/98 4-87☞If the image is not bright enough, it appears blue on the screen.Fig. 4-96
OPERATIONImage Optimization LSM 5104-88 B 40-051 e 07/98On the Channel Settings panel of the "Scan Control" window, set the PMT gain with
OPERATIONLSM 510 Image OptimizationB 40-051 e 07/98 4-894.7.2 Pinhole adjustment☞In all existing standard configurations, the pinholes have already
OPERATIONImage Optimization LSM 5104-90 B 40-051 e 07/98Click on the <Pinhole> button.This opens the "Pinhole & CollimatorControl&quo
OPERATIONLSM 510 Image OptimizationB 40-051 e 07/98 4-91Automatic pinhole adjustment and collimatorThe position of the pinhole (X-Y-Z-coordinates) i
NOTES ON DEVICE SAFETYLSM 510 Notes on handling the computer and data mediaB 40-051 e 07/98 1-51.4 Notes on handling the computer and data mediaThe
OPERATIONImage Optimization LSM 5104-92 B 40-051 e 07/98Click on the <Optimal Position> button in theCollimator box. Optimum positioning of th
OPERATIONLSM 510 Image OptimizationB 40-051 e 07/98 4-934.7.3 Scan speedThe signal-to-noise ratio can be substantiallyimproved by reducing the scann
OPERATIONImage Optimization LSM 5104-94 B 40-051 e 07/98Multiple-channelRequirements:The suitable lasers are on.The specimen has been positioned and
OPERATIONLSM 510 Image OptimizationB 40-051 e 07/98 4-95Click on the <Scan> button in the main menu.This opens the "Scan Control" wi
OPERATIONImage Optimization LSM 5104-96 B 40-051 e 07/98Click on the <Channels> button in the "Scan Control" window.This opens the C
OPERATIONLSM 510 Image OptimizationB 40-051 e 07/98 4-97Click on the <Display - Split xy> button of the image processing toolbar.This displays
OPERATIONImage Optimization LSM 5104-98 B 40-051 e 07/984.7.4 Channel Shift FunctionThe Channel Shift function is used to produce a congruent image
OPERATIONLSM 510 Image OptimizationB 40-051 e 07/98 4-99The following image shows the result of a pixel shift via the Shift function. This image cha
OPERATIONImage Optimization LSM 5104-100 B 40-051 e 07/98For overlaying fluorescence and transmitted-lightimages, click on the <Transmission>
OPERATIONLSM 510 Analysis of Images and StacksB 40-051 e 07/98 4-1014.8 Analysis of Images and StacksCall up an image just scanned, or an image fro
NOTES ON DEVICE SAFETYNotes on care, maintenance and service LSM 5101-6 B 40-051 e 07/981.5 Notes on care, maintenance and serviceThe manufacturer
OPERATIONAnalysis of Images and Stacks LSM 5104-102 B 40-051 e 07/98(1) Select - ChanYou can assign any color from the color palette to a channel, s
OPERATIONLSM 510 Analysis of Images and StacksB 40-051 e 07/98 4-103Zoom-+Enlarges the image by factor 2.Zoom-–Reduces the image by factor 2.Zoom 1:
OPERATIONAnalysis of Images and Stacks LSM 5104-104 B 40-051 e 07/98(3) Select - SliceThis function allows you to select and view individual slices
OPERATIONLSM 510 Analysis of Images and StacksB 40-051 e 07/98 4-105(4) Select - OverlayThis option makes available a number of measurement function
OPERATIONAnalysis of Images and Stacks LSM 5104-106 B 40-051 e 07/98(5) Select - ContrThis function allows you to vary the contrast and brightness o
OPERATIONLSM 510 Analysis of Images and StacksB 40-051 e 07/98 4-107(7) Select - AnimThis option allows you to call up the individual slice images o
OPERATIONAnalysis of Images and Stacks LSM 5104-108 B 40-051 e 07/98(9) Select - CropHere, you can define an area of any size, position and rotation
OPERATIONLSM 510 Analysis of Images and StacksB 40-051 e 07/98 4-109(10) Select - CopyCopies the image content to the clipboard, from where the imag
OPERATIONAnalysis of Images and Stacks LSM 5104-110 B 40-051 e 07/98(13) Display - xyAllows you to switch back to the original image from a differen
OPERATIONLSM 510 Analysis of Images and StacksB 40-051 e 07/98 4-111(15) Display - OrthoOrthogonal sections can be made anywhere in a stack. Further
NOTES ON DEVICE SAFETYLSM 510 Notes on handling the laser componentsB 40-051 e 07/98 1-71.6 Notes on handling the laser componentsThe LSM 510 is a
OPERATIONAnalysis of Images and Stacks LSM 5104-112 B 40-051 e 07/98By changing the parameters X, Y and Z on the Orthogonal Sections panel, the sect
OPERATIONLSM 510 Analysis of Images and StacksB 40-051 e 07/98 4-113You can reposition the (red) YZ plane in the same way using the symbol.The res
OPERATIONAnalysis of Images and Stacks LSM 5104-114 B 40-051 e 07/98(16) Display - CutIn a stack you can generate sections along a plane of freely s
OPERATIONLSM 510 Analysis of Images and StacksB 40-051 e 07/98 4-115(17) Display - GalleryThe various slices of a stack are shown in a tiled present
OPERATIONAnalysis of Images and Stacks LSM 5104-116 B 40-051 e 07/98Clicking on the <Data> button causes the Z data to be entered into the ima
OPERATIONLSM 510 Analysis of Images and StacksB 40-051 e 07/98 4-117(18) Display - 2.5DClicking on this button displays an image in a pseudo-3D mode
OPERATIONAnalysis of Images and Stacks LSM 5104-118 B 40-051 e 07/98(19) Display - HistoThe histogram function displays a graph of the intensity dis
OPERATIONLSM 510 Analysis of Images and StacksB 40-051 e 07/98 4-119(20) Display - ProfileThe Display-Profile function shows the intensity profile a
OPERATIONAnalysis of Images and Stacks LSM 5104-120 B 40-051 e 07/98You can place markings wherever you like and follow up this line detecting the i
OPERATIONLSM 510 Analysis of Images and StacksB 40-051 e 07/98 4-121(21) Display - Coloc.The Display-Coloc. function presents a comparison between t
NOTES ON DEVICE SAFETYWarning and information labels LSM 5101-8 B 40-051 e 07/981.7 Warning and information labelsThe warning and information label
OPERATIONAnalysis of Images and Stacks LSM 5104-122 B 40-051 e 07/98Fig. 4-130Identical images produce a clean diagonal line running from bottom lef
OPERATIONLSM 510 Analysis of Images and StacksB 40-051 e 07/98 4-123(22) Display - AreaClicking on the <Display-Area> button opens the Area Me
OPERATIONAnalysis of Images and Stacks LSM 5104-124 B 40-051 e 07/98Clicking on the <Flood fill> button (paint jar) and moving the cursor to t
OPERATIONLSM 510 Analysis of Images and StacksB 40-051 e 07/98 4-125If you specify a top and bottom intensity threshold, the area lying within this
OPERATIONAnalysis of Images and Stacks LSM 5104-126 B 40-051 e 07/98(23) Display - Prev.This function enables you to assemble a preview of all picto
OPERATIONLSM 510 Analysis of Images and StacksB 40-051 e 07/98 4-127Fig. 4-134Assembly ofimage,intensityprofile andscan info
OPERATIONAnalysis of Images and Stacks LSM 5104-128 B 40-051 e 07/98A layout generated with Prev. (Preview) can be printed by clicking on the <Pr
OPERATIONLSM 510 Analysis of Images and StacksB 40-051 e 07/98 4-129(24) Display - Info☞Clicking on the <Info> button, all parameters used to
OPERATIONAnalysis of Images and Stacks LSM 5104-130 B 40-051 e 07/98(25) 3D ViewFor the further three-dimensional analysis of image sequences (stack
OPERATIONLSM 510 Analysis of Images and StacksB 40-051 e 07/98 4-131☞The "Preview" function permits you to regard the influence of paramet
NOTES ON DEVICE SAFETYLSM 510 Warning and information labelsB 40-051 e 07/98 1-9Fig. 1-2 Warning and information labels on the Axioplan 2 microscope
OPERATIONAnalysis of Images and Stacks LSM 5104-132 B 40-051 e 07/98(b) ProjectionRequirement:A stack of images must be available.Clicking of the &l
OPERATIONLSM 510 Analysis of Images and StacksB 40-051 e 07/98 4-133☞The computed 3D sequence can be animated with the <Anim> button in the Se
OPERATIONAnalysis of Images and Stacks LSM 5104-134 B 40-051 e 07/98☞To view the computed 3D sequence as a gallery on the screen, click on the <D
OPERATIONLSM 510 Analysis of Images and StacksB 40-051 e 07/98 4-135(c) StereoRequirement:A stack of images must be available.Clicking on the <St
OPERATIONAnalysis of Images and Stacks LSM 5104-136 B 40-051 e 07/98To start computation of the stereoscopic image, click on the <Apply> butto
OPERATIONLSM 510 Analysis of Images and StacksB 40-051 e 07/98 4-137On clicking on the <Apply> button, the two stereo mates are presented side
OPERATIONDatabase / Loading and Storing of Images LSM 5104-138 B 40-051 e 07/984.9 Data base / Loading and Storing of imagesAll the generated image
OPERATIONLSM 510 Database / Loading and Storing of ImagesB 40-051 e 07/98 4-139Enter the name of the database you want to create in the File name te
OPERATIONDatabase / Loading and Storing of Images LSM 5104-140 B 40-051 e 07/98Click on the <Create> button.This creates the new image databas
OPERATIONLSM 510 Database / Loading and Storing of ImagesB 40-051 e 07/98 4-1414.9.2 Loading an image from databaseClick on the <File> button
INTRODUCTIONLSM 510II B 40-051 e 07/98Knowledge of this manual is required for the operation of the instrument. Would you therefore pleasemake yours
NOTES ON DEVICE SAFETYWarning and information labels LSM 5101-10 B 40-051 e 07/98Fig. 1-3 Warning and information labels on laser components (page 1
OPERATIONDatabase / Loading and Storing of Images LSM 5104-142 B 40-051 e 07/98This opens a window, e.g. Multi Channel 2_0.mdb - LSM 510, with butto
OPERATIONLSM 510 Database / Loading and Storing of ImagesB 40-051 e 07/98 4-143Click on the <Gallery> button.All images of the image database,
OPERATIONDatabase / Loading and Storing of Images LSM 5104-144 B 40-051 e 07/98Fig. 4-154Image processing and analysis can beeffected via the two-co
OPERATIONLSM 510 Database / Loading and Storing of ImagesB 40-051 e 07/98 4-1454.9.3 Saving an imageClick on the <File> button of the main me
OPERATIONDatabase / Loading and Storing of Images LSM 5104-146 B 40-051 e 07/98Enter the name of the image in the Name textbox, e.g. Spores.Click on
OPERATIONLSM 510 Database / Loading and Storing of ImagesB 40-051 e 07/98 4-147The "Single Channel 2_0.mdb-LSM510" window appears.The wind
OPERATIONDatabase / Loading and Storing of Images LSM 5104-148 B 40-051 e 07/984.9.4 Import of imagesClick on the <File> button of the main m
OPERATIONLSM 510 Database / Loading and Storing of ImagesB 40-051 e 07/98 4-1494.9.5 Export of imagesThe <Export> function allows the export
OPERATIONDatabase / Loading and Storing of Images LSM 5104-150 B 40-051 e 07/98Notes:System backupA complete backup is contained on the enclosed opt
OPERATIONLSM 510 MacroB 40-051 e 07/98 4-1514.10 MacroThe macro function permits the recording, running and editing of command sequences and theiral
NOTES ON DEVICE SAFETYLSM 510 Warning and information labelsB 40-051 e 07/98 1-11Fig. 1-3 Warning and information labels on laser components (page 2
OPERATIONMacro LSM 5104-152 B 40-051 e 07/98Fig. 4-164This window allows you to manage project data and to allocate macros to the buttons in the mai
OPERATIONLSM 510 MacroB 40-051 e 07/98 4-1534.10.3 Recording and running of macrosBefore recording a command sequence, you can enter the name for t
OPERATIONMacro LSM 5104-154 B 40-051 e 07/984.10.4 Assignment of macros to the macro buttons in the main windowPress the <Assign Macro to Button
OPERATIONLSM 510 MacroB 40-051 e 07/98 4-1554.10.5 Editing and debugging of macrosThe <Edit> button activates IDE which allows macros to be e
OPERATIONShut-Down Procedure LSM 5104-156 B 40-051 e 07/984.11 Shut-Down ProcedureNever shut down the computer by its main switch while your LSM pro
OPERATIONLSM 510 Shut-Down ProcedureB 40-051 e 07/98 4-1574.11.2 Running down the operating systemMove the cursor to the bottom margin of the scree
OPERATIONShut-Down Procedure LSM 5104-158 B 40-051 e 07/98This opens the "Shut Down Windows" window, in which you can select between Shut
OPERATIONLSM 510 Shut-Down ProcedureB 40-051 e 07/98 4-159The screen now displays the message"Shutdown in Progress - Please wait while the syst
OPERATIONAnnex LSM 5104-160 B 40-051 e 07/984.12 Annex4.12.1 Application-specific configurationsApplication-specific configurations for module 510-1
OPERATIONLSM 510 AnnexB 40-051 e 07/98 4-161Application-specific configurations for module 510-2 (488/568), 2 PMTsFITC (Cy2) Rhod (Cy3, TexRed) FITC
NOTES ON DEVICE SAFETYWarning and information labels LSM 5101-12 B 40-051 e 07/98
OPERATIONAnnex LSM 5104-162 B 40-051 e 07/98Application-specific configurations for module 510-3 (458/488, 543, 633), 3 PMTsLucifer Yellow FITC (Cy2
OPERATIONLSM 510 AnnexB 40-051 e 07/98 4-163Application-specific configurations for module 510-4 (488/568, 633), 3 PMTsFITC (Cy2) Rhod (Cy3, TexRed)
OPERATIONAnnex LSM 5104-164 B 40-051 e 07/98Application-specific configurations for module 510-5 (351/364, 458/488, 543), 3 PMTsDAPI Lucifer Yellow
OPERATIONLSM 510 AnnexB 40-051 e 07/98 4-165Application-specific configurations for module 510-6 (351/364, 458/488, 543, 633), 4 PMTsDAPI Lucifer Ye
OPERATIONAnnex LSM 5104-166 B 40-051 e 07/98DAPI/FITC/Rhod/Cy5DAPI (nb) FITC (nb) Rhodamine (nb)Laser364, 488, 543,633364 488 543HFTUV/488/543/633 U
OPERATIONLSM 510 AnnexB 40-051 e 07/98 4-167Application-specific configurations for module 510-7 (351/364, 488/568, 633), 4 PMTsDAPI FITC (Cy2) Rhod
OPERATIONAnnex LSM 5104-168 B 40-051 e 07/98DAPI/FITC/Rhod DAPI/FITC/Rhod/Cy5DAPI/FITC/Cy5 DAPI/Rhod/Cy5 FITC/Rhod/Cy5Laser364, 488, 568 364, 488, 5
OPERATIONLSM 510 AnnexB 40-051 e 07/98 4-169Application-specific configurations for module 510-8 (458/488, 543, 633), 2 PMTsLucifer Yellow FITC (Cy2
OPERATIONAnnex LSM 5104-170 B 40-051 e 07/98Application-specific configurations for module 510-9 (351/364, 458/488, 543), 2 PMTsDAPI Lucifer Yellow
OPERATIONLSM 510 AnnexB 40-051 e 07/98 4-1714.12.2 Filter change in the detection beam path of channels 1 and 2For optimum investigation of specime
LSM 510 - SETUP REQUIREMENTSLSM 510 ContentsB 40-051 e 07/98 2-1CHAPTER 2 LSM 510 - SETUP REQUIREMENTSCONTENTS2 LSM 510 - SETUP REQUIREMENTS...
OPERATIONAnnex LSM 5104-172 B 40-051 e 07/984.12.3 Detaching / Attaching the Scanning Module from / to Microscope StandsTool needed: 3 mm Allen key
OPERATIONLSM 510 AnnexB 40-051 e 07/98 4-173Fig. 4-171 Change-over of the Scanning Module
OPERATIONAnnex LSM 5104-174 B 40-051 e 07/984.12.4 Hints on the use of the HRZ 200 fine focusing stage4.12.4.1 General descriptionThe HRZ 200 fine
OPERATIONLSM 510 AnnexB 40-051 e 07/98 4-1754.12.4.3 OperationFine focusingSelect the <Acquire> button from the LSM 510 main menu.Select the &
OPERATIONAnnex LSM 5104-176 B 40-051 e 07/98Z Mode via the HRZ 200This mode is used to acquire two- or three-dimensional stacks of images in differe
OPERATIONLSM 510 AnnexB 40-051 e 07/98 4-177Clicking on the additional <Leveling> button moves the HRZ 200 to the zero position, while themoto
OPERATIONAnnex LSM 5104-178 B 40-051 e 07/98The shift is read off from the microscope stages. In the case of the manual Axioplan 2 stage, x can ber
OPERATIONLSM 510 AnnexB 40-051 e 07/98 4-1794.12.5 Scanning stagesThe following software description applies to systems which are equipped with a sc
OPERATIONAnnex LSM 5104-180 B 40-051 e 07/98XY Step SizeThe required step size for the scanning stage can be set in three ways:1.) by shifting the s
OPERATIONLSM 510 AnnexB 40-051 e 07/98 4-181<Z> MovesClicking on the Up arrow key moves the specimen stage/nosepiece upwards (the distance bet
LSM 510 - SETUP REQUIREMENTSContents LSM 5102-2 B 40-051 e 07/98
OPERATIONAnnex LSM 5104-182 B 40-051 e 07/984.12.5.2 Expert ModeThe following window opens in the Expert Mode after clicking on the Stage button in
OPERATIONLSM 510 AnnexB 40-051 e 07/98 4-183The Current Position and Selected Position display for X and Y is below.Below that, you will find the ta
OPERATIONAnnex LSM 5104-184 B 40-051 e 07/98<Zero>Zeros the Current Position display and thus sets the currently set stage position to 0 in re
OPERATIONLSM 510 AnnexB 40-051 e 07/98 4-1854.12.6 Specification of Trigger-Interface LSM510Application:With the LSM510 Release 2.01 you can contro
OPERATIONAnnex LSM 5104-186 B 40-051 e 07/98Trigger pulse description:Level detection:Low level: 0.0 - 1.0 VHigh level: 3.0 - 5.0 VSlew rate:rising
OPERATIONLSM 510 AnnexB 40-051 e 07/98 4-187Pin assignment:No. Name Direction Description1 Trig1O Out Trigger Output #12 Trig2O Out Trigger Output #
OPERATIONAnnex LSM 5104-188 B 40-051 e 07/984.12.7 MonitordiodeThe monitordiode is placed in the excitation ray path of the LSM 510 behind the beam
OPERATIONLSM 510 AnnexB 40-051 e 07/98 4-189Change to the "Scan Control" window and press <Cont.>; the system will scan with the dio
OPERATIONAnnex LSM 5104-190 B 40-051 e 07/98Examples of application:a) Checking the laser powerThis function is not automized so far. To qualitativ
OPERATIONLSM 510 AnnexB 40-051 e 07/98 4-191To use the monitordiode for ratio application the following steps have to be done:Activate the ratio cha
LSM 510 - SETUP REQUIREMENTSLSM 510 Space RequirementsB 40-051 e 07/98 2-32 LSM 510 - SETUP REQUIREMENTS2.1 Space Requirements2.1.1 LSM (one micr
OPERATIONAnnex LSM 5104-192 B 40-051 e 07/98The following image is an example of the reduction of correlated noise. The low frequency noise hasbeen
OPERATIONLSM 510 AnnexB 40-051 e 07/98 4-193The single steps to find the right setting of all the parameters to be set are listed in the following:A
OPERATIONAnnex LSM 5104-194 B 40-051 e 07/98Amplifier GainThe diode signal is set to the right range (graylevel between 50 and 200 - 8bit image / 75
OPERATIONLSM 510 AnnexB 40-051 e 07/98 4-195Gain and offset in Ratio channelIf the setting of the PMT channel is finished, the range of the ratio ch
OPERATIONAnnex LSM 5104-196 B 40-051 e 07/98Default settings are 150 and 3000 respectively. With the help of the range indicator the default value i
INTRODUCTION TO LASER SCANNING MICROSCOPYLSM 510 ContentsB 40-051 e 07/98 5-1CHAPTER 5 3D FOR LSM 510CONTENTSPage5 3D FOR LSM 510...
INTRODUCTION TO LASER SCANNING MICROSCOPYContents LSM 5105-2 B 40-051 e 07/98
INTRODUCTION TO LASER SCANNING MICROSCOPYLSM 510 Overview and ExplanationsB 40-051 e 07/98 5-35 3D FOR LSM 5105.1 Overview and Explanations5.1.1
INTRODUCTION TO LASER SCANNING MICROSCOPYOverview and Explanations LSM 5105-4 B 40-051 e 07/98Image sequences can consist of several channels. Most
INTRODUCTION TO LASER SCANNING MICROSCOPYLSM 510 User InterfaceB 40-051 e 07/98 5-55.2 User Interface5.2.1 IntroductionThis section describes the fo
LSM 510 - SETUP REQUIREMENTSSpace Requirements LSM 5102-4 B 40-051 e 07/982.1.3 LSM with Ar laser (UV) and two microscopes: 450 x 220 cm☞We recomme
INTRODUCTION TO LASER SCANNING MICROSCOPYUser Interface LSM 5105-6 B 40-051 e 07/98Display window This window is used to display image sequences.Dis
INTRODUCTION TO LASER SCANNING MICROSCOPYLSM 510 User InterfaceB 40-051 e 07/98 5-75.2.2 Main WindowThe Main window includes:the Menuthe Tool barand
INTRODUCTION TO LASER SCANNING MICROSCOPYUser Interface LSM 5105-8 B 40-051 e 07/98Edit MenuCopy Copies the contents of the Display window to the cl
INTRODUCTION TO LASER SCANNING MICROSCOPYLSM 510 User InterfaceB 40-051 e 07/98 5-9View MenuSet Channel Colour The colour and the weight of the sing
INTRODUCTION TO LASER SCANNING MICROSCOPYUser Interface LSM 5105-10 B 40-051 e 07/98GalleryThe Gallery is used as an overview of the images availabl
INTRODUCTION TO LASER SCANNING MICROSCOPYLSM 510 User InterfaceB 40-051 e 07/98 5-11To start the automatic animation of an image sequence start the
INTRODUCTION TO LASER SCANNING MICROSCOPYUser Interface LSM 5105-12 B 40-051 e 07/98Control Element of the PlayerThe three arrow shaped controls on
INTRODUCTION TO LASER SCANNING MICROSCOPYLSM 510 User InterfaceB 40-051 e 07/98 5-13Set Channel ColourThis function sets the colour and weight for t
INTRODUCTION TO LASER SCANNING MICROSCOPYFunctions LSM 5105-14 B 40-051 e 07/985.3 Functions5.3.1 Functions in the File MenuOpen ImageThis function
INTRODUCTION TO LASER SCANNING MICROSCOPYLSM 510 FunctionsB 40-051 e 07/98 5-15Carl Zeiss Vision image sequences must have a number digit at the end
LSM 510 - SETUP REQUIREMENTSLSM 510 Power RequirementsB 40-051 e 07/98 2-52.2 Power Requirements☞The LSM 510 comes with a mains power supply cord a
INTRODUCTION TO LASER SCANNING MICROSCOPYFunctions LSM 5105-16 B 40-051 e 07/98Save Image AsThis function saves an image or image sequence to disk o
INTRODUCTION TO LASER SCANNING MICROSCOPYLSM 510 FunctionsB 40-051 e 07/98 5-17The content of the Gallery is shown in the Input section. The selecti
INTRODUCTION TO LASER SCANNING MICROSCOPYFunctions LSM 5105-18 B 40-051 e 07/98PrintThis function prints the current Display window contents.The sta
INTRODUCTION TO LASER SCANNING MICROSCOPYLSM 510 FunctionsB 40-051 e 07/98 5-195.3.2 Functions in the Edit MenuCopyThis function copies the current
INTRODUCTION TO LASER SCANNING MICROSCOPYFunctions LSM 5105-20 B 40-051 e 07/98Edit ChannelsThis function allows to add or to remove channels to a s
INTRODUCTION TO LASER SCANNING MICROSCOPYLSM 510 FunctionsB 40-051 e 07/98 5-21On the Delete Channel tab sheet channels of the Input 1 image sequenc
INTRODUCTION TO LASER SCANNING MICROSCOPYFunctions LSM 5105-22 B 40-051 e 07/985.3.3 Functions in the Process MenuArithmetics - AddThis function ad
INTRODUCTION TO LASER SCANNING MICROSCOPYLSM 510 FunctionsB 40-051 e 07/98 5-23This function adds the two image sequences Input 1 and Input 2 voxel
INTRODUCTION TO LASER SCANNING MICROSCOPYFunctions LSM 5105-24 B 40-051 e 07/98Arithmetics - SubtractThis function subtracts two image sequences.Fig
INTRODUCTION TO LASER SCANNING MICROSCOPYLSM 510 FunctionsB 40-051 e 07/98 5-251 - Wrap No normalization - the grey values are displayed modulo 256
LSM 510 - SETUP REQUIREMENTSPower Requirements / Physical Dimensions LSM 5102-6 B 40-051 e 07/982.2.1 Phase 1 (LSM)feeds the following units:Laser
INTRODUCTION TO LASER SCANNING MICROSCOPYFunctions LSM 5105-26 B 40-051 e 07/98Contrast - InteractiveThis function allows interactive changes of the
INTRODUCTION TO LASER SCANNING MICROSCOPYLSM 510 FunctionsB 40-051 e 07/98 5-27A grey value range of the Input image sequence is scaled to another r
INTRODUCTION TO LASER SCANNING MICROSCOPYFunctions LSM 5105-28 B 40-051 e 07/98Entering a new value in the appropriate text boxes, clicking on the b
INTRODUCTION TO LASER SCANNING MICROSCOPYLSM 510 FunctionsB 40-051 e 07/98 5-29Contrast - AutomaticThis function scales the grey values of an image
INTRODUCTION TO LASER SCANNING MICROSCOPYFunctions LSM 5105-30 B 40-051 e 07/98Output defines the name of the result sequence. It will get only thos
INTRODUCTION TO LASER SCANNING MICROSCOPYLSM 510 FunctionsB 40-051 e 07/98 5-31Contrast – LinearizeThis function scales a range of grey values of an
INTRODUCTION TO LASER SCANNING MICROSCOPYFunctions LSM 5105-32 B 40-051 e 07/98The Output histogram shows the resulting histogram. The horizontal ax
INTRODUCTION TO LASER SCANNING MICROSCOPYLSM 510 FunctionsB 40-051 e 07/98 5-33Smooth (Gauss)This function performs a Gauss filter.Fig. 5-16The nois
INTRODUCTION TO LASER SCANNING MICROSCOPYFunctions LSM 5105-34 B 40-051 e 07/98MorphologyThe following four functions perform basic operations of ma
INTRODUCTION TO LASER SCANNING MICROSCOPYLSM 510 FunctionsB 40-051 e 07/98 5-35The input image sequence is analyzed voxel by voxel with a selected s
LSM 510 - SETUP REQUIREMENTSLSM 510 Dimensions ... / Envirenment Requirements / VibrationsB 40-051 e 07/98 2-72.4 Dimension of shipment cratesCrat
INTRODUCTION TO LASER SCANNING MICROSCOPYFunctions LSM 5105-36 B 40-051 e 07/98Sequential image:Z-2Z-1ZZ+1Z+2Volume view: Cube cross shape: createdt
INTRODUCTION TO LASER SCANNING MICROSCOPYLSM 510 FunctionsB 40-051 e 07/98 5-37The result of erosion and dilation is called opening. On the one hand
INTRODUCTION TO LASER SCANNING MICROSCOPYFunctions LSM 5105-38 B 40-051 e 07/98Morphology - ErodeThis function erodes structures in an image sequenc
INTRODUCTION TO LASER SCANNING MICROSCOPYLSM 510 FunctionsB 40-051 e 07/98 5-39Parameters:Input Input image sequenceOutput Resulting image sequenceS
INTRODUCTION TO LASER SCANNING MICROSCOPYFunctions LSM 5105-40 B 40-051 e 07/98The following shapes (numbered 1 to 3 from left to right) are availab
INTRODUCTION TO LASER SCANNING MICROSCOPYLSM 510 FunctionsB 40-051 e 07/98 5-41Morphology - OpenThis function carries out an opening.Fig. 5-22In the
INTRODUCTION TO LASER SCANNING MICROSCOPYFunctions LSM 5105-42 B 40-051 e 07/98Parameters:Input Input image sequenceOutput Resulting image sequenceS
INTRODUCTION TO LASER SCANNING MICROSCOPYLSM 510 FunctionsB 40-051 e 07/98 5-43Morphology - CloseThis function carries out a closing.Fig. 5-23In the
INTRODUCTION TO LASER SCANNING MICROSCOPYFunctions LSM 5105-44 B 40-051 e 07/98Parameters:Input Input image sequenceOutput Resulting image sequenceS
INTRODUCTION TO LASER SCANNING MICROSCOPYLSM 510 FunctionsB 40-051 e 07/98 5-45Segment - InteractiveThis function carries out a grey value segmentat
INTRODUCTIONLSM 510B 40-051 e 07/98 IIIDeveloped in Collaboration with the European Molecular Biology Laboratory (EMBL)PF 102209Meyerhofstr. 1D-6901
LSM 510 - SETUP REQUIREMENTSLaser Specifications LSM 5102-8 B 40-051 e 07/982.7 Laser Specifications2.7.1 Coherent Enterprise 653 II: 352, 364 nm,
INTRODUCTION TO LASER SCANNING MICROSCOPYFunctions LSM 5105-46 B 40-051 e 07/98The Green and Blue/Red option buttons of the parameter Colour determi
INTRODUCTION TO LASER SCANNING MICROSCOPYLSM 510 FunctionsB 40-051 e 07/98 5-47Segment - AutomaticThe function carries out an automatic grey value s
INTRODUCTION TO LASER SCANNING MICROSCOPYFunctions LSM 5105-48 B 40-051 e 07/98If Blue/Red is selected, the voxels with grey values within the inter
INTRODUCTION TO LASER SCANNING MICROSCOPYLSM 510 FunctionsB 40-051 e 07/98 5-49Boolean - AndThis function carries out a bit-by-bit And calculation f
INTRODUCTION TO LASER SCANNING MICROSCOPYFunctions LSM 5105-50 B 40-051 e 07/98Boolean - OrThis function carries out a bit-by-bit Or calculation for
INTRODUCTION TO LASER SCANNING MICROSCOPYLSM 510 FunctionsB 40-051 e 07/98 5-51Boolean - XorThis function carries out a bit-by-bit Xor calculation f
INTRODUCTION TO LASER SCANNING MICROSCOPYFunctions LSM 5105-52 B 40-051 e 07/98Boolean - NotThis function carries out a bit-by-bit negation of an im
INTRODUCTION TO LASER SCANNING MICROSCOPYLSM 510 FunctionsB 40-051 e 07/98 5-53Boolean - MaskThis function masks a grey value image sequence.Fig. 5-
INTRODUCTION TO LASER SCANNING MICROSCOPYFunctions LSM 5105-54 B 40-051 e 07/98ScrapThis function deletes or selects objects in a specified size ran
INTRODUCTION TO LASER SCANNING MICROSCOPYLSM 510 FunctionsB 40-051 e 07/98 5-55Fill HolesThis function fills holes in all objects.Fig. 5-32All holes
LSM 510 - SETUP REQUIREMENTSLSM 510 Dimensions ... / Envirenment Requirements / VibrationsB 40-051 e 07/98 2-92.7.4 LASOS LGK 7812 ML-1/LGN 7812:
INTRODUCTION TO LASER SCANNING MICROSCOPYFunctions LSM 5105-56 B 40-051 e 07/985.3.4 Functions in the View MenuRender - SurfaceThis function displa
INTRODUCTION TO LASER SCANNING MICROSCOPYLSM 510 FunctionsB 40-051 e 07/98 5-57MethodThe Input sequence defines the data to be reconstructed. If it
INTRODUCTION TO LASER SCANNING MICROSCOPYFunctions LSM 5105-58 B 40-051 e 07/98Use the parameter Reflection to control the ratio of diffuse and refl
INTRODUCTION TO LASER SCANNING MICROSCOPYLSM 510 FunctionsB 40-051 e 07/98 5-59Render - Surface: Method DescriptionThis method displays the surface
INTRODUCTION TO LASER SCANNING MICROSCOPYFunctions LSM 5105-60 B 40-051 e 07/98Render - AlphaThis function displays an image sequence according to t
INTRODUCTION TO LASER SCANNING MICROSCOPYLSM 510 FunctionsB 40-051 e 07/98 5-61MethodThe Input sequence defines the data to be reconstructed. If it
INTRODUCTION TO LASER SCANNING MICROSCOPYFunctions LSM 5105-62 B 40-051 e 07/98Application1. This method can be applied, if the structures in the In
INTRODUCTION TO LASER SCANNING MICROSCOPYLSM 510 FunctionsB 40-051 e 07/98 5-63Render - Alpha: Method DescriptionEach Output pixel is a weighted sum
INTRODUCTION TO LASER SCANNING MICROSCOPYFunctions LSM 5105-64 B 40-051 e 07/985.3.5 Functions in the Measurement MenuMeasurement ConceptMeasuremen
INTRODUCTION TO LASER SCANNING MICROSCOPYLSM 510 FunctionsB 40-051 e 07/98 5-65Measurement ProcessThe measurement process consists of three steps: r
LSM 510 - SETUP REQUIREMENTSLaser Specifications LSM 5102-10 B 40-051 e 07/982.8 MicroscopesInverted Axiovert 100 M BP or SPUpright Axioplan 2 MOTAl
INTRODUCTION TO LASER SCANNING MICROSCOPYFunctions LSM 5105-66 B 40-051 e 07/98Automatic Object Measurement – Object FeaturesA measurement feature d
INTRODUCTION TO LASER SCANNING MICROSCOPYLSM 510 FunctionsB 40-051 e 07/98 5-67The dialog shows two lists. One shows the Available Features as group
INTRODUCTION TO LASER SCANNING MICROSCOPYFunctions LSM 5105-68 B 40-051 e 07/98Object Features (geometric)If Object Features are selected, one set o
INTRODUCTION TO LASER SCANNING MICROSCOPYLSM 510 FunctionsB 40-051 e 07/98 5-69Object Features (densitometric)Group Name Name DescriptionMean Densit
INTRODUCTION TO LASER SCANNING MICROSCOPYFunctions LSM 5105-70 B 40-051 e 07/98Automatic Object Measurement - Volume FeaturesA measurement feature d
INTRODUCTION TO LASER SCANNING MICROSCOPYLSM 510 FunctionsB 40-051 e 07/98 5-71Parameters:Available Features List of available object featuresSelect
INTRODUCTION TO LASER SCANNING MICROSCOPYFunctions LSM 5105-72 B 40-051 e 07/98Volume Features (geometric)The volume-related measurement generates o
INTRODUCTION TO LASER SCANNING MICROSCOPYLSM 510 FunctionsB 40-051 e 07/98 5-73Automatic Object Measurement - ConditionThe measurement conditions ar
INTRODUCTION TO LASER SCANNING MICROSCOPYFunctions LSM 5105-74 B 40-051 e 07/98The list on the very left at the dialog shows all the measurement Fea
INTRODUCTION TO LASER SCANNING MICROSCOPYLSM 510 FunctionsB 40-051 e 07/98 5-75Automatic Object Measurement - GeneralThis function carries out an au
LSM 510 - SETUP REQUIREMENTSLSM 510 Microscopes / Scanning ModuleB 40-051 e 07/98 2-112.10 Laser Module VISSingle-mode polarization preserving fib
INTRODUCTION TO LASER SCANNING MICROSCOPYFunctions LSM 5105-76 B 40-051 e 07/98A single object of interest can be visualized. Clicking on a specific
LSM 510 - SETUP REQUIREMENTSLaser Module VIS / Laser Module UV LSM 5102-12 B 40-051 e 07/98
INTRODUCTION TO LASER SCANNING MICROSCOPYLSM 510 ContentsB 40-051 e 07/98 3-1CHAPTER 3 INTRODUCTION TOLASER SCANNING MICROSCOPYCONTENTS3 INTRODUCTIO
INTRODUCTION TO LASER SCANNING MICROSCOPYContents LSM 5103-2 B 40-051 e 07/98
INTRODUCTION TO LASER SCANNING MICROSCOPYLSM 510 Principle of Laser Scanning MicroscopyB 40-051 e 07/98 3-33 INTRODUCTION TO LASER SCANNING MICROSC
INTRODUCTION TO LASER SCANNING MICROSCOPYThree-Dimensional Presentation of LSM Image Stacks LSM 5103-4 B 40-051 e 07/98In order to obtain an image o
INTRODUCTION TO LASER SCANNING MICROSCOPYLSM 510 Three-Dimensional Presentation of LSM Image StacksB 40-051 e 07/98 3-5Orthogonal sectionsThis compu
INTRODUCTIONLSM 510IV B 40-051 e 07/98
INTRODUCTION TO LASER SCANNING MICROSCOPYOptical Diagram of the LSM 510 LSM 5103-6 B 40-051 e 07/983.3 Optical Diagram of the LSM 510 (Schematic)Fi
INTRODUCTION TO LASER SCANNING MICROSCOPYLSM 510 Performance Features of the LSM 510B 40-051 e 07/98 3-73.4 Performance Features of the LSM 5103.4.
INTRODUCTION TO LASER SCANNING MICROSCOPYPerformance Features of the LSM 510 LSM 5103-8 B 40-051 e 07/98The scanning field size can be freely select
INTRODUCTION TO LASER SCANNING MICROSCOPYLSM 510 Performance Features of the LSM 510B 40-051 e 07/98 3-9e) The analyzer slider for conventional DIC
INTRODUCTION TO LASER SCANNING MICROSCOPYPerformance Features of the LSM 510 LSM 5103-10 B 40-051 e 07/98(3) Transmitted-light illuminationa) The il
INTRODUCTION TO LASER SCANNING MICROSCOPYLSM 510 Performance Features of the LSM 510B 40-051 e 07/98 3-113.4.3 Computer hardware and softwareThe LS
INTRODUCTION TO LASER SCANNING MICROSCOPYPerformance Features of the LSM 510 LSM 5103-12 B 40-051 e 07/98
INTRODUCTION TO LASER SCANNING MICROSCOPYLSM 510 ContentsB 40-051 e 07/98 3-1CHAPTER 3 INTRODUCTION TOLASER SCANNING MICROSCOPYCONTENTS3 INTRODUCTIO
INTRODUCTION TO LASER SCANNING MICROSCOPYContents LSM 5103-2 B 40-051 e 07/98
INTRODUCTION TO LASER SCANNING MICROSCOPYLSM 510 Principle of Laser Scanning MicroscopyB 40-051 e 07/98 3-33 INTRODUCTION TO LASER SCANNING MICROSC
INTRODUCTIONLSM 510B 40-051 e 07/98 VHow to make best use of the LSM 510 operating instructionsA few symbols in these operating instructions will he
INTRODUCTION TO LASER SCANNING MICROSCOPYThree-Dimensional Presentation of LSM Image Stacks LSM 5103-4 B 40-051 e 07/98In order to obtain an image o
INTRODUCTION TO LASER SCANNING MICROSCOPYLSM 510 Three-Dimensional Presentation of LSM Image StacksB 40-051 e 07/98 3-5Orthogonal sectionsThis compu
INTRODUCTION TO LASER SCANNING MICROSCOPYOptical Diagram of the LSM 510 LSM 5103-6 B 40-051 e 07/983.3 Optical Diagram of the LSM 510 (Schematic)Fi
INTRODUCTION TO LASER SCANNING MICROSCOPYLSM 510 Performance Features of the LSM 510B 40-051 e 07/98 3-73.4 Performance Features of the LSM 5103.4.
INTRODUCTION TO LASER SCANNING MICROSCOPYPerformance Features of the LSM 510 LSM 5103-8 B 40-051 e 07/98The scanning field size can be freely select
INTRODUCTION TO LASER SCANNING MICROSCOPYLSM 510 Performance Features of the LSM 510B 40-051 e 07/98 3-9e) The analyzer slider for conventional DIC
INTRODUCTION TO LASER SCANNING MICROSCOPYPerformance Features of the LSM 510 LSM 5103-10 B 40-051 e 07/98(3) Transmitted-light illuminationa) The il
INTRODUCTION TO LASER SCANNING MICROSCOPYLSM 510 Performance Features of the LSM 510B 40-051 e 07/98 3-113.4.3 Computer hardware and softwareThe LS
INTRODUCTION TO LASER SCANNING MICROSCOPYPerformance Features of the LSM 510 LSM 5103-12 B 40-051 e 07/98
OPERATIONLSM 510 ContentsB 40-051 e 07/98 4-1CHAPTER 4 OPERATIONCONTENTSPage4 OPERATION ...
INTRODUCTIONLSM 510VI B 40-051 e 07/98Scope of Equipment SuppliedCountry: ...Order number: ...
OPERATIONContents LSM 5104-2 B 40-051 e 07/98Page4.7 Image optimization...
OPERATIONLSM 510 General / SoftwareB 40-051 e 07/98 4-34 OPERATION4.1 GeneralThis section describes the operation of the LSM 510 Laser Scanning Mic
OPERATIONSoftware LSM 5104-4 B 40-051 e 07/984.2.1 Boot WINDOWS NT☞Drive "A" must not contain a diskette.Normally, the LSM system is turn
OPERATIONLSM 510 SoftwareB 40-051 e 07/98 4-5WINDOWS NT operating system is being loaded.The "Begin Logon" WINDOWS dialog box appears on
OPERATIONSoftware LSM 5104-6 B 40-051 e 07/98After you have made the two entries, confirm them by clicking on the <OK> button or hitting the&l
OPERATIONLSM 510 Quick startB 40-051 e 07/98 4-74.3 Quick start4.3.1 Starting the LSM Program(1) From the Windows NT operating system desktop dou
OPERATIONQuick start LSM 5104-8 B 40-051 e 07/984.3.2 Creating a data base for image storage(1) Click on the <File> button from LSM 510 tool
OPERATIONLSM 510 Quick startB 40-051 e 07/98 4-94.3.3 Turning the Lasers On(1) Click on the <Acquire> button from the LSM 510 tool bar.(2)
OPERATIONQuick start LSM 5104-10 B 40-051 e 07/984.3.4 Look in the Microscope and Visually Set Up Your Specimen(1) Click on the <Micro> and
OPERATIONLSM 510 Quick startB 40-051 e 07/98 4-11(2) Move the silver slider on the side of the microscope to the appropriate position - the correc
INTRODUCTIONLSM 510B 40-051 e 07/98 VIIKodak XLS 8650 PS printer 417735 9003-000Transparency exposure device (D) 412410 9011-000Large system table 4
OPERATIONQuick start LSM 5104-12 B 40-051 e 07/98(7) Note that there is a course/fine control button on the side of the microscope next to the focu
OPERATIONLSM 510 Quick startB 40-051 e 07/98 4-13(2) Click on the <Recording Configurations> button in the "Configuration Control"
OPERATIONQuick start LSM 5104-14 B 40-051 e 07/984.3.6 Laser Scanning(1) Click on the <Scan> button from the LSM 510 tool bar.The "Scan
OPERATIONLSM 510 Quick startB 40-051 e 07/98 4-15(3) Click on the <Single> button on the right side of the Scan Control menu.(4) For multi-
OPERATIONQuick start LSM 5104-16 B 40-051 e 07/98(9) To make the image brighter or dimmer, adjust Detector Gain. This adjustment is very sensitive.
OPERATIONLSM 510 Quick startB 40-051 e 07/98 4-17(12) Once you have optimized a particular channel, you can switch to the next channel desired and
OPERATIONQuick start LSM 5104-18 B 40-051 e 07/98(17) To further improve image quality you can slow the scan rate allowing more photons to integrat
OPERATIONLSM 510 Quick startB 40-051 e 07/98 4-194.3.7 Z SectioningOnce you have set up your image as defined in the above section, you can collect
OPERATIONQuick start LSM 5104-20 B 40-051 e 07/98(5) Click on the <XY cont.> button to begin scanning.(6) Move the focus down manually (cloc
OPERATIONLSM 510 Quick startB 40-051 e 07/98 4-21(8) Click on the <Stop> button.(9) Click on the <Optimal Interval> button in the &qu
INTRODUCTIONLSM 510VIII B 40-051 e 07/981This section contains general notes on device safety, safe operation, and possible hazardscaused by failure
OPERATIONOverview of Menu Items LSM 5104-22 B 40-051 e 07/984.4 Overview of the Menu ItemsStart the LSM program as follows:Double-click on the Star
OPERATIONLSM 510 Overview of Menu ItemsB 40-051 e 07/98 4-23Operating modesStart Routine ModeClick on this button if you want to work with pre-conf
OPERATIONOverview of Menu Items LSM 5104-24 B 40-051 e 07/98Click on the <Start Expert Mode> button.The "LSM 510 - Expert Mode" main
OPERATIONLSM 510 Overview of Menu ItemsB 40-051 e 07/98 4-25Process Used for the mathematical integration of acquired images.<Shift> allows y
OPERATIONOverview of Menu Items LSM 5104-26 B 40-051 e 07/98Options For custom-configuration of software and hardware options, and forexporting syst
OPERATIONLSM 510 Image Acquisition (Acquire)B 40-051 e 07/98 4-274.5 Image Acquisition (Acquire)In the main menu toolbar, click on <Acquire>
OPERATIONImage Acquisition (Acquire) LSM 5104-28 B 40-051 e 07/984.5.1 Laser settingsClick on the <Laser> button.This opens the "Laser Co
OPERATIONLSM 510 Image Acquisition (Acquire)B 40-051 e 07/98 4-29To switch on the Enterprise laser, proceed as follows:(1) The internal water cool
OPERATIONImage Acquisition (Acquire) LSM 5104-30 B 40-051 e 07/984.5.2 Microscope settings (conventional microscopy)Place specimen on microscope st
OPERATIONLSM 510 Image Acquisition (Acquire)B 40-051 e 07/98 4-31With Transmitted Light activated , the halogen lamp is automatically occluded in
INTRODUCTIONLSM 510B 40-051 e 07/98 IX1Notes on Device Safety2LSM 510 Setup Requirements3Introduction to Laser Scanning Microscopy4Operation5Softwar
OPERATIONImage Acquisition (Acquire) LSM 5104-32 B 40-051 e 07/98If you want to use an objective which is not contained in the nosepiece, proceed as
OPERATIONLSM 510 Image Acquisition (Acquire)B 40-051 e 07/98 4-33(4) Click on the <Maintain> button in the main menu and then on the <Rebo
OPERATIONImage Acquisition (Acquire) LSM 5104-34 B 40-051 e 07/984.5.2.1 Transmitted-light observation (Axioplan 2 MOT)Push in the silver slider (4
OPERATIONLSM 510 Image Acquisition (Acquire)B 40-051 e 07/98 4-354.5.2.2 Epi-fluorescence observation (Axioplan 2 MOT)Turn on the HBO 100 W power
OPERATIONImage Acquisition (Acquire) LSM 5104-36 B 40-051 e 07/98Other filter sets:The filter sets described in this section are included in the sta
OPERATIONLSM 510 Image Acquisition (Acquire)B 40-051 e 07/98 4-374.5.2.3 Transmitted-light observation (Axiovert 100 M BP)Pull out the silver slid
OPERATIONImage Acquisition (Acquire) LSM 5104-38 B 40-051 e 07/984.5.2.4 Epi-fluorescence observation (Axiovert 100 M BP)Turn on the HBO 50 power s
OPERATIONLSM 510 Image Acquisition (Acquire)B 40-051 e 07/98 4-394.5.3 Beam path / ConfigurationClick on the <Config> button.This opens the &
OPERATIONImage Acquisition (Acquire) LSM 5104-40 B 40-51 e 07/984.5.3.1 Taking over the configuration with Configuration ControlAfter clicking on t
OPERATIONLSM 510 Image Acquisition (Acquire)B 40-051 e 07/98 4-41Click on the desired configuration.The selected configuration is shown in the Confi
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