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INTRODUCTION TO LASER SCANNING MICROSCOPY
Overview and Explanations LSM 510
5-4 B 40-051 e 07/98
Image sequences can consist of several channels. Most functions and the Display window are providing
buttons to select all or a subset of channels stored in the selected image sequence. The Output image
sequence will only get those channels which are selected on the input side. The button selects all
channels in the image sequence to be used clicking with the left mouse button on it.
Clicking with the left mouse button on any of the number buttons toggles the state of this single
channel.
Clicking with the right mouse button on any of the number buttons selects this single channel
exclusively. All other channels are deselected.
5.1.2 The Image Properties
Every image sequence has its own set of properties. They contain the scaling and the scaling units. The
scaling and its units are required for 3D reconstruction and measurement. If a sequence of LSM-TIFF
images is read in, the image properties are loaded automatically from the file header and allocated to the
image properties of the new image sequence.
5.1.3 Memory Usage
All images shown in the Gallery are currently loaded in the system memory of the operating system.
Some functions need additional temporarily used memory during their execution.
If the memory is running low delete some images from the Gallery. If the images are needed afterwards
they must be saved to disk first. Normally all functions produce a new result (output) image sequence. In
order to save some memory, other image sequences currently presented in the Gallery can be selected
as result position. The output image is overwritten by entry execution of a function.
- Laser Scanning Microscope 1
- B 40-051 e 07/98 III 3
- IV B 40-051 e 07/98 4
- Scope of Equipment Supplied 6
- INTRODUCTION 10
- X B 40-051 e 07/98 10
- CONTENTS 11
- NOTES ON DEVICE SAFETY 12
- Contents LSM 510 12
- 1-2 B 40-051 e 07/98 12
- 1 NOTES ON DEVICE SAFETY 13
- Exit aperture 18
- B 40-051 e 07/98 1-9 19
- 1-10 B 40-051 e 07/98 20
- LGK 7774 21
- LGK 7628-1 21
- 1-12 B 40-051 e 07/98 22
- LSM 510 - SETUP REQUIREMENTS 24
- 2-2 B 40-051 e 07/98 24
- Fig. 2-1 25
- Fig. 2-2 25
- Fig. 2-3 26
- Fig. 2-4 27
- 2-12 B 40-051 e 07/98 34
- CHAPTER 3 INTRODUCTION TO 35
- LASER SCANNING MICROSCOPY 35
- 3-2 B 40-051 e 07/98 36
- Module VIS 40
- Scan ModuleMicroscope 40
- Module UV 40
- 3-12 B 40-051 e 07/98 46
- CHAPTER 4 OPERATION 59
- 4 OPERATION 61
- Fig. 4-1 62
- Fig. 4-2 63
- Fig. 4-3 63
- Fig. 4-4 64
- Fig. 4-5 65
- Fig. 4-6 66
- Fig. 4-7 66
- Fig. 4-8 67
- Fig. 4-9 67
- Fig. 4-10 68
- Fig. 4-11 69
- Fig. 4-12 70
- Fig. 4-13 71
- Fig. 4-14 72
- Fig. 4-15 73
- Fig. 4-16 74
- Fig. 4-17 75
- Fig. 4-18 76
- Fig. 4-19 77
- Fig. 4-20 78
- Fig. 4-21 79
- Fig. 4-22 80
- Fig. 4-23 82
- Fig. 4-24 82
- Fig. 4-25 83
- Fig. 4-26 83
- Fig. 4-27 83
- Fig. 4-28 84
- Fig. 4-29 84
- Fig. 4-30 85
- Fig. 4-31 86
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- Fig. 4-33 89
- Fig. 4-34 90
- Fig. 4-35 90
- Fig. 4-36 91
- Fig. 4-37 92
- Fig. 4-38 93
- Fig. 4-39 95
- Fig. 4-40 96
- Fig. 4-41 97
- Fig. 4-42 98
- Fig. 4-43 100
- Fig. 4-44 101
- Fig. 4-45 103
- OPERATION 104
- 4-46 B 40-51 e 07/98 104
- B 40-051 e 07/98 4-47 105
- Fig. 4-48 106
- Fig. 4-49 107
- Fig. 4-50 108
- Fig. 4-51 109
- Fig. 4-52 110
- Fig. 4-53 111
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- Fig. 4-55 113
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- Fig. 4-57 115
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- Fig. 4-60 117
- Fig. 4-61 118
- Fig. 4-62 119
- Fig. 4-63 120
- Fig. 4-64 121
- Fig. 4-65 122
- Fig. 4-66 123
- B 40-051 e 07/98 4-67 125
- Fig. 4-68 126
- Colored marker positions 126
- Fig. 4-69 127
- Fig. 4-70 128
- Fig. 4-71 129
- Fig. 4-72 129
- Fig. 4-73 130
- Fig. 4-74 130
- Fig. 4-75 131
- Fig. 4-76 131
- Fig. 4-77 132
- Fig. 4-78 133
- Fig. 4-79 133
- Fig. 4-81 134
- Fig. 4-80 134
- Fig. 4-82 135
- Fig. 4-83 135
- Fig. 4-90 139
- Fig. 4-91 140
- LSM 510 Image Optimization 141
- B 40-051 e 07/98 4-83 141
- Fig. 4-93 142
- Fig. 4-94 143
- Fig. 4-95 144
- B 40-051 e 07/98 4-87 145
- Fig. 4-97 146
- Fig. 4-98 147
- Fig. 4-99 148
- Fig. 4-100 149
- Fig. 4-101 150
- Fig. 4-102 151
- Fig. 4-103 152
- Fig. 4-104 153
- / Ampl. Gain 154
- Fig. 4-105 155
- Fig. 4-106 156
- Fig. 4-107 157
- Fig. 4-108 158
- Fig. 4-109 159
- Fig. 4-110 160
- Fig. 4-111 161
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- 4-124 B 40-051 e 07/98 182
- Fig. 4-133 183
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- Fig. 4-140 190
- Fig. 4-141 191
- 4-134 B 40-051 e 07/98 192
- Fig. 4-143 193
- Fig. 4-144 194
- B 40-051 e 07/98 4-137 195
- Fig. 4-146 196
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- Fig. 4-168 216
- Fig. 4-169 217
- LSM 510 Annex 219
- B 40-051 e 07/98 4-161 219
- Annex LSM 510 220
- 4-162 B 40-051 e 07/98 220
- B 40-051 e 07/98 4-163 221
- 4-164 B 40-051 e 07/98 222
- B 40-051 e 07/98 4-165 223
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- B 40-051 e 07/98 4-167 225
- 4-168 B 40-051 e 07/98 226
- B 40-051 e 07/98 4-169 227
- 4-170 B 40-051 e 07/98 228
- Fig. 4-170 Filter change 229
- Allen key 230
- B 40-051 e 07/98 4-173 231
- Fine focusing 233
- Fig. 4-173 234
- Fig. 4-174 237
- Fig. 4-175 240
- Fig. 4-176 246
- Fig. 4-177 247
- Fig. 4-178 249
- Fig. 4-179 250
- Fig. 4-180 252
- Fig. 4-181 253
- CHAPTER 5 3D FOR LSM 510 255
- 5-2 B 40-051 e 07/98 256
- Positive rotation directions 257
- Fig. 5-1 257
- Fig. 5-2 259
- Display window 260
- Fig. 5-4 265
- Fig. 5-5 267
- Fig. 5-6 268
- Fig. 5-7 270
- Fig. 5-9 274
- Fig. 5-10 275
- Fig. 5-11 276
- Fig. 5-12 278
- Fig. 5-13 280
- Fig. 5-14 283
- Fig. 5-15 285
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- Fig. 5-18 291
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- Fig. 5-24 299
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- Fig. 5-27 304
- Fig. 5-28 305
- Fig. 5-29 306
- Fig. 5-30 307
- Fig. 5-31 308
- Fig. 5-32 309
- Fig. 5-33 310
- Fig. 5-34 314
- Functions LSM 510 318
- 5-64 B 40-051 e 07/98 318
- Measurement Concept 318
- Fig. 5-36 319
- Fig. 5-37 320
- Fig. 5-39 327
- Measured 329
- Object Features 329
- Volume Features 329
Verwandte Produkte und Handbücher für Mikroskope Zeiss LSM 510 META
Mikroskope Zeiss N HBO 103 Spezifikationen
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(120 Seiten)
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