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INTRODUCTION TO LASER SCANNING MICROSCOPY
LSM 510 Functions
B 40-051 e 07/98 5-29
Contrast - Automatic
This function scales the grey values of an image sequence to the maximum possible range.
Fig. 5-14
The Automatic tab sheet of the Contrast dialog window must be selected.
This function enhances the contrast of an image sequence by spreading the grey value distribution over
the maximum possible range. This function is used to achieve a better view of an image.
The light and dark grey value ranges with a low share of pixels are excluded from the operation by the
parameter Threshold. The Threshold units are in thousandths of the total number of voxels. Using a
value of 10 means that the scale interval is set so that 5/1000 of the total number of voxels on the light
side, and 5/1000 of the total number of voxels on the dark side of the grey value distribution are
excluded.
Input indicates the sequence to enhance. If it is a multichannel sequence, a single channel, all channels,
or any number can be selected. The Input histogram shows the grey value distribution of the selected
channels of the Input image sequence.
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