Zeiss LSM 510 META Spezifikationen Seite 93
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OPERATION
LSM 510 Image Acquisition (Acquire)
B 40-051 e 07/98 4-35
4.5.2.2 Epi-fluorescence observation (Axioplan 2
MOT
)
Turn on the HBO 100 W power supply with switch (4-37/2).
Push in the silver slider (4-37/8) on the microscope tube as far as it will go.
This opens the light path for specimen observation through the eyepieces.
Switch on the reflected light.
Pull out the occluding slider (4-37/1) to a light-passing position; actuate shutter switch (4-37/4) for
reflected light if it is in transmitted-light position.
☞
To avoid excessive bleaching, expose the specimen to the minimum possible irradiation, i.e.
keep the irradiation time as short as possible.
In the Reflector Turret list box, select the reflector module (filter sets) to suit the type of fluorescence
excitation. Proceed as follows:
Open the Reflector Turret list box.
Click on the desired reflector module.
The reflector turret moves the selected
reflector module into the beam path.
☞
The FITC filter set (FSET09) consists
of an excitation filter for the
450 - 490 nm spectral range, an
FT color splitter for 510 nm and an
LP long pass filter, which passes
emission light wavelengths greater
than 510 nm.
Fig. 4-38
- Laser Scanning Microscope 1
- B 40-051 e 07/98 III 3
- IV B 40-051 e 07/98 4
- Scope of Equipment Supplied 6
- INTRODUCTION 10
- X B 40-051 e 07/98 10
- CONTENTS 11
- NOTES ON DEVICE SAFETY 12
- Contents LSM 510 12
- 1-2 B 40-051 e 07/98 12
- 1 NOTES ON DEVICE SAFETY 13
- Exit aperture 18
- B 40-051 e 07/98 1-9 19
- 1-10 B 40-051 e 07/98 20
- LGK 7774 21
- LGK 7628-1 21
- 1-12 B 40-051 e 07/98 22
- LSM 510 - SETUP REQUIREMENTS 24
- 2-2 B 40-051 e 07/98 24
- Fig. 2-1 25
- Fig. 2-2 25
- Fig. 2-3 26
- Fig. 2-4 27
- 2-12 B 40-051 e 07/98 34
- CHAPTER 3 INTRODUCTION TO 35
- LASER SCANNING MICROSCOPY 35
- 3-2 B 40-051 e 07/98 36
- Module VIS 40
- Scan ModuleMicroscope 40
- Module UV 40
- 3-12 B 40-051 e 07/98 46
- CHAPTER 4 OPERATION 59
- 4 OPERATION 61
- Fig. 4-1 62
- Fig. 4-2 63
- Fig. 4-3 63
- Fig. 4-4 64
- Fig. 4-5 65
- Fig. 4-6 66
- Fig. 4-7 66
- Fig. 4-8 67
- Fig. 4-9 67
- Fig. 4-10 68
- Fig. 4-11 69
- Fig. 4-12 70
- Fig. 4-13 71
- Fig. 4-14 72
- Fig. 4-15 73
- Fig. 4-16 74
- Fig. 4-17 75
- Fig. 4-18 76
- Fig. 4-19 77
- Fig. 4-20 78
- Fig. 4-21 79
- Fig. 4-22 80
- Fig. 4-23 82
- Fig. 4-24 82
- Fig. 4-25 83
- Fig. 4-26 83
- Fig. 4-27 83
- Fig. 4-28 84
- Fig. 4-29 84
- Fig. 4-30 85
- Fig. 4-31 86
- Fig. 4-32 88
- Fig. 4-33 89
- Fig. 4-34 90
- Fig. 4-35 90
- Fig. 4-36 91
- Fig. 4-37 92
- Fig. 4-38 93
- Fig. 4-39 95
- Fig. 4-40 96
- Fig. 4-41 97
- Fig. 4-42 98
- Fig. 4-43 100
- Fig. 4-44 101
- Fig. 4-45 103
- OPERATION 104
- 4-46 B 40-51 e 07/98 104
- B 40-051 e 07/98 4-47 105
- Fig. 4-48 106
- Fig. 4-49 107
- Fig. 4-50 108
- Fig. 4-51 109
- Fig. 4-52 110
- Fig. 4-53 111
- Fig. 4-54 112
- Fig. 4-55 113
- Fig. 4-56 114
- Fig. 4-57 115
- Fig. 4-58 115
- Fig. 4-59 116
- Fig. 4-60 117
- Fig. 4-61 118
- Fig. 4-62 119
- Fig. 4-63 120
- Fig. 4-64 121
- Fig. 4-65 122
- Fig. 4-66 123
- B 40-051 e 07/98 4-67 125
- Fig. 4-68 126
- Colored marker positions 126
- Fig. 4-69 127
- Fig. 4-70 128
- Fig. 4-71 129
- Fig. 4-72 129
- Fig. 4-73 130
- Fig. 4-74 130
- Fig. 4-75 131
- Fig. 4-76 131
- Fig. 4-77 132
- Fig. 4-78 133
- Fig. 4-79 133
- Fig. 4-81 134
- Fig. 4-80 134
- Fig. 4-82 135
- Fig. 4-83 135
- Fig. 4-90 139
- Fig. 4-91 140
- LSM 510 Image Optimization 141
- B 40-051 e 07/98 4-83 141
- Fig. 4-93 142
- Fig. 4-94 143
- Fig. 4-95 144
- B 40-051 e 07/98 4-87 145
- Fig. 4-97 146
- Fig. 4-98 147
- Fig. 4-99 148
- Fig. 4-100 149
- Fig. 4-101 150
- Fig. 4-102 151
- Fig. 4-103 152
- Fig. 4-104 153
- / Ampl. Gain 154
- Fig. 4-105 155
- Fig. 4-106 156
- Fig. 4-107 157
- Fig. 4-108 158
- Fig. 4-109 159
- Fig. 4-110 160
- Fig. 4-111 161
- Fig. 4-112 162
- Fig. 4-113 163
- Fig. 4-114 164
- Fig. 4-115 164
- Fig. 4-116 165
- Fig. 4-117 166
- Fig. 4-118 168
- Fig. 4-119 169
- Fig. 4-120 170
- Fig. 4-121 171
- Fig. 4-122 172
- Fig. 4-123 173
- Fig. 4-124 174
- Fig. 4-125 175
- Fig. 4-126 176
- Fig. 4-127 177
- Fig. 4-128 178
- Fig. 4-129 179
- Fig. 4-130 180
- Fig. 4-131 181
- 4-124 B 40-051 e 07/98 182
- Fig. 4-133 183
- Fig. 4-134 185
- Fig. 4-135 186
- Fig. 4-136 187
- Fig. 4-137 188
- Fig. 4-138 188
- Fig. 4-139 189
- Fig. 4-140 190
- Fig. 4-141 191
- 4-134 B 40-051 e 07/98 192
- Fig. 4-143 193
- Fig. 4-144 194
- B 40-051 e 07/98 4-137 195
- Fig. 4-146 196
- Fig. 4-147 196
- Fig. 4-148 198
- Fig. 4-149 199
- Fig. 4-150 199
- Fig. 4-151 199
- Fig. 4-152 200
- Fig. 4-153 201
- Fig. 4-154 202
- Fig. 4-155 203
- Fig. 4-156 203
- Fig. 4-157 204
- Fig. 4-158 205
- Fig. 4-159 206
- Fig. 4-160 206
- Fig. 4-161 207
- Fig. 4-162 207
- Fig. 4-163 209
- Fig. 4-164 210
- Fig. 4-165 212
- Fig. 4-166 214
- Fig. 4-167 215
- Fig. 4-168 216
- Fig. 4-169 217
- LSM 510 Annex 219
- B 40-051 e 07/98 4-161 219
- Annex LSM 510 220
- 4-162 B 40-051 e 07/98 220
- B 40-051 e 07/98 4-163 221
- 4-164 B 40-051 e 07/98 222
- B 40-051 e 07/98 4-165 223
- 4-166 B 40-051 e 07/98 224
- B 40-051 e 07/98 4-167 225
- 4-168 B 40-051 e 07/98 226
- B 40-051 e 07/98 4-169 227
- 4-170 B 40-051 e 07/98 228
- Fig. 4-170 Filter change 229
- Allen key 230
- B 40-051 e 07/98 4-173 231
- Fine focusing 233
- Fig. 4-173 234
- Fig. 4-174 237
- Fig. 4-175 240
- Fig. 4-176 246
- Fig. 4-177 247
- Fig. 4-178 249
- Fig. 4-179 250
- Fig. 4-180 252
- Fig. 4-181 253
- CHAPTER 5 3D FOR LSM 510 255
- 5-2 B 40-051 e 07/98 256
- Positive rotation directions 257
- Fig. 5-1 257
- Fig. 5-2 259
- Display window 260
- Fig. 5-4 265
- Fig. 5-5 267
- Fig. 5-6 268
- Fig. 5-7 270
- Fig. 5-9 274
- Fig. 5-10 275
- Fig. 5-11 276
- Fig. 5-12 278
- Fig. 5-13 280
- Fig. 5-14 283
- Fig. 5-15 285
- Fig. 5-16 287
- Fig. 5-17 288
- Fig. 5-18 291
- Fig. 5-19 291
- Fig. 5-20 292
- Fig. 5-21 293
- Fig. 5-22 295
- Fig. 5-23 297
- Fig. 5-24 299
- Fig. 5-25 301
- Fig. 5-26 303
- Fig. 5-27 304
- Fig. 5-28 305
- Fig. 5-29 306
- Fig. 5-30 307
- Fig. 5-31 308
- Fig. 5-32 309
- Fig. 5-33 310
- Fig. 5-34 314
- Functions LSM 510 318
- 5-64 B 40-051 e 07/98 318
- Measurement Concept 318
- Fig. 5-36 319
- Fig. 5-37 320
- Fig. 5-39 327
- Measured 329
- Object Features 329
- Volume Features 329
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