
INTRODUCTION TO LASER SCANNING MICROSCOPY
LSM 510 Functions
B 40-051 e 07/98 5-45
Segment - Interactive
This function carries out a grey value segmentation by means of thresholding.
Fig. 5-24
The Interactive tab sheet of Segment dialog window must be selected.
Segmentation is especially used to generate binary regions. These are required for the measurement.
Two threshold values determine which grey value range of the Input image sequence is preserved
and/or deleted in the Output image sequence. Only one channel of a multichannel sequence can be
selected as Input. Output will always be a single channel sequence.
The vertical scaling of the histogram can be adjusted with the scroll bar at the right edge of the
histogram. This setting has no influence on the function.
The thresholds Low and High are determined either by moving the borderlines in the grey value
histogram or by the scroll bars underneath. Furthermore, the values for Low, Center and High can be set
through entry in the corresponding fields.
To move the lower (L) and upper (H) thresholds at the same time, move the vertical line in the grey value
histogram or set the scroll bar (C).
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