
Confocal Z Sectioning
Number of Sections for correct sampling
Optical thickness d depends on:
• Wavelength
λ
• Objective lens, N.A.
• Refractive index n
• Pinhole diameter P
d ~ P n λ / (N.A)
2
~ 0.5 µm @ 63x1.4
Optimal: (no missing information @ minimal
number of sections)
Slices overlap by the half of their thickness
„Nyquist-“ or Sampling- Theorem
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