Zeiss LSM 510 META Bedienungsanleitung PDF herunterladen (Seite 17)

LSM 510 Meta Training Notes

Nyquist Sampling

z In order to preserve the spatial resolution in an image the sampling rate

should be twice that of the maximum frequency.

z Adjust the optical zoom in order to achieve the optimal x,y pixel size.

Objective

Numerical

Aperture

Immersion

Coverslip

Thickness

(mm)

Working

Distance

(mm)

Resolution

(μm) (1

Airy Unit)

*Optimal

Z-Step

(μm)

**XY

Resolution

(μm)

*Optimal

XY pixel

size

***Optimal

Zoom

40x C-

Apochromat

1.2 Water .14-.18 .22 .7 .35 .25 .13 3.5

40x Plan-

Apochromat

.95 Air .31 - .21 .16 .8 .41 .31 .16 2.9

63x Plan-

Apochromat

1.4 Oil .17 .18 .6 .29 .21 .11 2.5

100x Plan-

Apochromat

1.4 Oil .17 .09 .6 .29 .21 .11 1.6

10x Plan-

Neofluar

.3 Air .17 5.6 9.0 4.48 1.0 .5 3.6

20x Plan-

Neofluar

.5 Air .17 2.0 3.2 1.59 .60 .3 3.0

63x Plan-

Neofluar

1.25 Oil .17 .10 .7 .37 .24 .12 2.3

10x Fluar .5 Air .17 2.0 3.2 1.59 .60 .3 5.9

40x Fluar 1.3 Oil .17 .14 .7 .34 .23 .12 3.7

1 2 ... 12 13 14 15 16 17 18 19 20 21

LSM 510 Meta 1
Training Notes 1
LSM 510 Meta Training Notes 10

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