Zeiss LSM 510 META Bedienungsanleitung PDF herunterladen (Seite 2)

Zeiss LSM 510 Meta Confocal Microscope Instructions

W123 Parsons Hall 23 Academic Way, Durham, NH 03824

Phone: 603-862-2790 Fax: 603-862-4104

www.unh.edu/research/uic

Basic Image Acquisition

9. Click Laser, Config, Scan, and Stage buttons.

The four windows that open will generally be left open throughout your session.

10. Laser Control. We have three lasers: the Argon laser can produce four lines of excitation (458, 477, 488,

514 nm); the two Helium/Neon lasers each produce a single line of excitation (543 nm, 633 nm).

 ONLY TURN A LASER ON IF IT HAS BEEN ALLOWED TO COOL FOR AT LEAST 45 MIN

SINCE IT WAS LAST TURNED OFF (consult slip of paper left in front of Hg lamp power supply).

Turn on only the laser(s) you need (depending on the fluor(s) in your sample) by first highlighting the

appropriate laser(s) (click on it).

For the Ar laser, click Standby button and then, when Status reads Ready, click On button. Increase

Output (%) with slider until Tube Current reads 6.1 A. Hold down Ctrl on keypad while clicking

arrowheads at either end of slider for finer increments of change. Output (%) will be ~ 40-50.

For the two He/Ne lasers, once highlighted, click the On button.

11. Configuration Control: Single Track Option.

a. In general, if imaging:

a single fluor

two fluors with widely separated emission spectra (such that the

Ar and HeNe633 lasers will likely be used for excitation)

leave Channel Mode and Single Track selected. Otherwise, proceed

to step 12.

b. Click Config button.

11a

1 2 3 4 5 6 7 ... 12 13

Keine Kommentare

Zeiss LSM 510 META Bedienungsanleitung Seite 2