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INSTRUMENT DESCRIPTION Axioskop 40
Carl Zeiss Objectives Axioskop 40 FL
1-8 B 40-810 e 12/01
1.4 Objectives
The objectives are the optical heart of the
microscope. The following is an example of how
objectives can be labelled:
Achroplan 10x/0.25 ∞/0.17
where
10x : objective magnification, with a defined
color ring on the objective being allocated
to each magnification step (Zeiss color
code)
0.25 : numerical aperture
∞ : infinite image distance; these objectives
can only be used with ICS microscopes
from Carl Zeiss
0.17 : can only be used with cover slip thickness
D = 0.17 mm
or
− : can be used with cover slip thickness D = 0
or 0.17 mm
Other labels:
Oil : oil immersion objective
Ph 2 : phase contrast objective with a green color ring and phase stop Ph 2
Color ring code for objective magnification:
Color ring on
objective
black brown red orange yellow green light blue dark blue white
Magnification
factor
1.25x 2.5x 4x; 5x 6.3x 10x 16x;20x;
25x;32x
40x; 50x 63x 100x;
150x
Objective magnification multiplied by eyepiece magnification (usually 10x) results in overall visual
magnification: e.g. 10 x 10 = 100x.
When working with the microscopes, total magnification should not drop below or exceed the range of
useful magnification. The range of useful magnification was defined by Ernst ABBE as 500 to 1,000
times the numerical aperture of the objective used . There is no resolution for further details above that
limit. Accordingly, the range of useful magnification for an objective with a numerical aperture of 0.25 is
between 125x and 250x.
Exact observance of the cover slip thickness of 0.17 mm is all the more necessary the higher the
numerical aperture of the objective. For this reason, certain objectives can be set to different cover slip
thicknesses (due to correction mount). To this end, a specimen area is searched for, and the position of
the correction ring where optimum focus and image contrast are obtained is determined (refocusing is
invariably required).
Fig. 1-1 Objective
- Operating Manual 1
- Axioskop 40 / Axioskop 40 FL 1
- Routine microscope 1
- INTRODUCTION Axioskop 40 12
- 0-12 B 40-810 e 12/01 12
- Overall view of Axioskop 40 12
- Axioskop 40 INTRODUCTION 13
- B 40-810 e 12/01 0-13 13
- Carl Zeiss Axioskop 40 FL 14
- 0-14 B 40-810 e 12/01 14
- INSTRUMENT DESCRIPTION 15
- 1 INSTRUMENT DESCRIPTION 16
- Axioskop 40 18
- 1-4 B 40-810 e 12/01 18
- 40 / 40 FL 18
- 1-6 B 40-810 e 12/01 20
- 1-16 B 40-810 e 12/01 30
- START-UP 31
- 2 START-UP 34
- Fig. 2-3 Inserting eyepieces 35
- Fig. 2-7 Mounting objectives 38
- Fig. 2-10 Axioskop 40 41
- (front and back) 41
- Fig. 2-16 Adjustment guide 44
- Fig. 2-21 Attaching HBO 50 47
- Fig. 2-22 Adjusting HBO 50 47
- Fig. 2-32 Changing the burner 56
- Fig. 2-33 Setting the torque 58
- Axioskop 40 START-UP 71
- B 40-810 e 12/01 2-41 71
- 4 Binocular tube 9 Plug 73
- OPERATION 75
- Axioskop 40 OPERATION 77
- Axioskop 40 FL Carl Zeiss 77
- B 40-810 e 12/01 3-3 77
- 3 OPERATION 78
- Key to Fig. 3-1: 79
- Fig. 3-3 Condenser 0.9/1.25 H 81
- Fig. 3-6 Specimen marker 83
- 3-10 B 40-810 e 12/01 84
- Fig. 3-8 Universal condenser 87
- (3-7/21) 88
- Fig. 3-15 Overview adapter 92
- Fig. 3-16 Filter mount 92
- Axioskop 40 FL 109
- (e.g. Contax 167 ARIA) 111
- (e.g. MC 80 112
- Video adapter 115
- (Cat. No.) 115
- Suitable for Comments 115
- OPERATION Axioskop 40 116
- 3-42 B 40-810 e 12/01 116
- Handycam DCR-PC100" 117
- Camera DSC-S70" 118
- 3-48 B 40-810 e 12/01 122
- 3-50 B 40-810 e 12/01 124
- Carl Zeiss Axioskop 40 FL 128
- 3-54 B 40-810 e 12/01 128
- Carl Zeiss 129
- Fig. 4-1 Changing fuses 131
- 4-10 B 40-810 e 12/01 138
- APPENDIX 139
- APPENDIX Axioskop 40 146
- A-8 B 40-810 e 12/01 146
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