Zeiss ZS-Series Bedienungsanleitung

ZeissZeiss LSM 510 LSM 510 ConfocalConfocal MicroscopeMicroscopeTraining NotebookTraining NotebookThe Center for NanotechnologyThe Center for Nanotech

F Acquire a ConfocalImageF. Acquire a ConfocalImageThe confocal can be used in SINGLE TRACK (one or several channels acquired SIMULTANEOUSLY), or MUL

F1 A i I i Si l T k M dF-1. Acquire an Image in Single Track Mode1) Select the CONFIG button on the main toolbar. 3) Select CONFIG. This will d

F2 A i I i Mlti T k MdF-2. Acquire an Image in Multi Track Mode1) Select the CONFIG button on the main toolbar. 3) Select CONFIG. This will displ

G1 Collect an ImageG-1. Collect an Image1) FIND opens a new image window and automatically pre-adjusts detector sensitivity.2) Use FAST XY for continu

G. Image Optimization G. Image Optimization G-2. Adjust Channel Settings1) Make sure theLSM2) Select theSCAN1) Make sure the LSM and ACQUIRE button

G3 O ti i D t t S ttiG-3. Optimize Detector Settings1) Select PALETTE.2) This opensColor Palette ListSelectRange2) This opens Color Palette List.

G4 Optimize Scanning ParametersG-4. Optimize Scanning Parameters1) Select MODE.2)Objective Lens & Image3) Line Step. Scans every nth line2) Objec

G4 Optimize Pinhole (Advanced)G-4. Optimize Pinhole (Advanced)For greatest signal and resolution, realignment of the pinhole for each filter combinat

G5 Adj t O ti l ZG-5. Adjust Optical ZoomThe scanner zoom allows you to "zoom in" on a region of interest and collect a magnified image. Thi

H Save DataH. Save Data1) Saving Individual Images– Collect one image.– In the image window, click SAVE AS.–In the windowSave Image andIn the window S

A Turn on the SystemA. Turn on the System1) Turn ON the FluoroArc mercury lamp. (Power supply is located under the vibration table)Thesimplest rule

I Acquire a Zseries StackI. Acquire a Z-series StackI-1. Mark First/Last Option1) Select the Z STACK button under Z SETTINGS.2) Select Z SLICE to ope

I2 Z Stii OtiI-2. Z –Sectioning Option1) Select theZSTACKbutton underZ1) Select the Z STACKbutton under Z SETTINGS.2) LINE SEL will collect an XY im

J Acq ire a Time SeriesJ. Acquire a Time SeriesBefore collecting a Time Series, set all parameters for collecting either a single XY section or a Z-st

k. Collect a DIC Imagek. Collect a DIC ImageK-1. Set up Kohler Illumination1) Select VIS mode on main toolbar and then focus sample.2) In BF step down

K2 I i VIS d d PMTK-2. Image in VIS mode and PMT1) S l t dDIC II f 20X DIC III f 40X d 63X1) Select condenser –DIC II for 20X, DIC III for 40X an

L Sh t D L. Shut Down 1) Select ACQUIRE. Open the Laser Control panel and turn OFF the HeNelasers. To turn off the Argon laser first click STANDBY, t

B Start Operating SoftwareB. Start Operating Software1) Select SCAN NEW IMAGES(otherwise software will work as a viewer only)viewer only).2) Click ST

C T th LC. Turn on the Lasers1) S l tACQUIRE1) Select ACQUIRE.2) Select LASER (opens laser control panel).3) Click STANDBY for the Argon laser (

D S t U D t bD. Set up a User Database1) S l tFILE1) Select FILE.2) Click NEW. In the Create New Database window, select drive G:\. Ct dit if

E Vi S i t th Mi (VIS d )E. View Specimen at the Microscope (VIS mode)1) Select ACQUIRE, and then click MICROon main toolbar. 2) Toggle betw

E1 L d S l t th MiE-1. Load Sample at the Microscope1) Make sure objective is lowered (use focus buttons) before putting a slide on the stage.Cond

E2 Vi S i i T itt d Li ht (B i ht Fi ld)E-2. View Specimen using Transmitted Light (Bright Field)1) Click the VIS button. This will automatic

E3 Vi S i i Fl R fl ti BF E-3. View Specimen using Fluorescence or Reflective BF Make sure the VIS button is selected and the Microscope Cont