Zeiss LSM 510 META Bedienungsanleitung Seite 44

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Carl Zeiss - Training Application and Support Center
Zeiss LSM 510 META - Guided Tour
44
Scanning a Z-Series using Mark First/Last
1) Select Z Stack
2) Start scanning using Fast XY or XY cont
3) Keep your eye on the image and move the
focus to the beginning of the Z-Series, then
select Mark First
4) Move the focus back in the opposite direction
to the end of the Z-Series, then select Mark Last
5) X:Y:Z = 1:1:1 sets the Z-interval so that the
voxel has identical dimensions in X, Y, and Z.
6) Start will initiate the acquisition of the Z-
Stack. The acquisition can be stopped at any
time.
NOTE
Focusing can be achieved manually (preferred),
or using Stage in the LSM menu if there is a
motorized scanning table.
Focus
Increment
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