Zeiss LSM 510 Bedienungsanleitung PDF herunterladen (Seite 18)

9. Acquiring a Z-series

Having set the system to acquire a satisfactory image, you can acquire a z-series. It may

be worthwhile changing the frame size to 512×512 to minimize file size and to speed

acquisition.

Click on the Z-Stack

button in the Scan control window.

Using Fast scan

mode to acquire a continuous image of the

specimen. Focus up and down with the

microscope focus wheel to ensure your

specimen is in the centre of the field of view

and the microscope is focused in the middle of

the specimen.

Stop acquisition.

You can now precisely define the middle and

depth of you z-series.

Ensure the Num Slices = 20 and the

Interval = 1 µm at this point to ensure the top

and bottom of the specimen is located.

Click the Range

button from the Scan control window – this

will generate a side view of you specimen.

This button is not available if the

MarkFirst/Last button is depressed.

(LEFT) The green line represents the

middle of your z-series. The upper red

line represents the top and the lower red

line the bottom of the z-series.

(RIGHT) Drag the green line to the

centre of the specimen and the red lines

to just outside the top and bottom of the

specimen.

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Zeiss LSM 510 Bedienungsanleitung Seite 18