Zeiss LSM 510 Bedienungsanleitung PDF herunterladen (Seite 20)

10. Advanced Options

10.1. Single-Track – simultaneous acquisition

Multi-track can solve the problem of cross-talk. Typically this occurs with bleed

through of green fluorescence in to the red channel. Multi Track avoids this by acquiring

the fluorescent channels sequentially, not simultaneously as with Single Track

acquisition. In Multi Track mode the red channel detector is turned on, the green one

off. Then the red fluorophore is imaged.

The red channel detector is then turned off, the green one on and the green fluorophore

imaged. Any bleed through of fluorescence from the green fluorophore to the red detector

does not register.

This switching between tracks means that there is a delay between channels. Sometimes

this is unacceptable, in particular for live cell imaging where the cell can move between

channels creating artefacts. In this case Single track mode should be used.

• To load pre-designated configurations

click the Config button in the

Configuration control window.

10.1.1. Getting the display ready

For multi-label experiments, it is best to have both channels

displayed at once.

To do this:

In the Live image window’s Display side-bar, click the Split

xy button

This will make your image small so resize the image window

(usual Windows mouse-drag). Then click on the Zoom button to

bring up the Zoom sidebar and select Auto button.

10.1.2. Optimise settings

Unlike Sequential acquisition (Multi-Track), each channel is acquired simultaneously

during optimization – so be quick!

Refer to section 7.2 for instructions on how to optimize the channel settings.

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Zeiss LSM 510 Bedienungsanleitung Seite 20