Zeiss LSM 510 Bedienungsanleitung PDF herunterladen (Seite 19)

In the Scan control window, click the Z-

slice button to bring up the Optical Slice

dialog

Click the Optimal Interval

button. Check the optical section

for each channel is the same and close. If you optical

sections are different for each wavelength go to

Mode/Channels and adjust the Pinhole so that each

channel has the same optical section – around 1 Airy

unit, but one channel will have to be slightly larger.

Readjust the red lines that indicate the top and bottom of the specimen (Step 7 above).

Reduce the Frame Size also if possible to speed stack-acquisition.

To acquire your z-series stack click the Start button.

The system will begin

scanning the specimen.

You can check progress by

selecting Gallery button

on the image window

sidebar.

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Zeiss LSM 510 Bedienungsanleitung Seite 19