Zeiss Axiovert 135M Spezifikationen PDF herunterladen (Seite 44)

Axiovert 25, Axiovert 25 C, Axiovert 25 CFL

08/99 B 40Ć012 e

2Ć10

2.4 Illumination and contrasting techniques

The description/application of illumination/contrasting techniques is based on the following

microscope settings:

• Axiovert 25 microscope is switched on.

• The "Illuminance" control (1Ć6ā/ā25) is in center position.

• The "beam path" switch (1Ć6ā/ā30) is in the position for observation.

2.4.1 Use of condensers

Condenser 0.2

0.4 0.55

Free distance between

condenser and stage

surface

 90 mm  53 mm  31 mm

plus 6 mm height

travel

Objective magnification 5x, 10x, 20x 5x, 10x, 20x, 32x, 40x 5x, 10x, 20x, 32x, 40x

Technique brightfield

phase contrast

inclined illumination

brightfield

phase contrast

VAREL contrast

(up to 32x)

inclined illumination

brightfield

phase contrast

VAREL contrast

inclined illumination

• To fully illuminate object fields even when lowĆpower objectives (< 5x) are used, push condenser

(2Ć14/3) out of its work position (dashed line in Fig. 2Ć15).

• When particularly high culture vessels are used, this position of the condenser is also required

in order to increase the free working distance to approx. 190 mm.

NOTE Only simple general illumination is obtained if no condenser is used!

• When using the condenser again for illumination, slide it exactly up to the stop position.

NOTE Loosening the three screws (2Ć15/7) of the condenser changer permits the condenser

0.4 to be replaced with the condenser 0.2 or 0.55.

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Zeiss Axiovert 135M Spezifikationen Seite 44