Zeiss Axiovert 135M Spezifikationen PDF herunterladen (Seite 46)

Axiovert 25, Axiovert 25 C, Axiovert 25 CFL

08/99 B 40Ć012 e

2Ć12

2.4.3 Phase contrast

• Preparations as described in section  2.4 and

2.4.1.

NOTE PhaseĆcontrast objectives are

labeled in green.

• Swing phaseĆcontrast objective (2Ć15/3) in

the beam path.

• Open the aperture diaphragm (2Ć15ā/ā2)

entirely.

• Insert the auxiliary lens (2Ć15/4) in one of the

tubes instead of an eyepiece and make the

phase rings of the objective visible by

focusing the eyelens.

• The phase ring of the phaseĆcontrast

objective appears as a gray ring in the bright

pupil (2Ć16/A).

Figure 2Ć16 Illustration of phaseĆcontrast adjustment

• Slide the annular diaphragm (2Ć17/1) of the Ph/H/Ph slider (2Ć15/1) matching the objective and

the inserted condenser in the beam path.

The phase ring of the objective appears centrally in the field of view, while the bright annular

stop may be out of center (2Ć16/B).

• Use the centering screws (2Ć17/3) on the Ph/H/Ph slider to move the phase ring and the annular

diaphragm until they are flush.

The gray phase ring of the objective must cover the bright ring stop completely (2Ć16/C).

Figure 2Ć15 Viewing in phase contrast

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Zeiss Axiovert 135M Spezifikationen Seite 46