Zeiss Axiolab A Bedienerhandbuch PDF herunterladen (Seite 46)

Carl Zeiss Axiolab A

3-4 B 40-015 e 06/99

3.2

Illumination and Contrasting Techniques

The description of the illumination and contrasting techniques is based on the following microscope

settings:

• The Axiolab A microscope is ready for operation as described in chapter 2 and switched on as

described in section 3.1.

• The pushrod (3-2/3) has been pushed in to direct 100% of the light to the observation port of the

binocular tube.

3.2.1

Setting of Reflected-Light Brightfield

• Place a specimen of as much contrast as

possible (3-2/6) on the specimen stage (3-2/10);

if required by the specimen height, lower the

specimen stage via the coarse drive (3-2/9) or

secure it in the lower stop position.

• Pull out plane glass pushrod (3-2/2).

• Look through the binocular tube (3-2/1) and set

the eyepiece distance and interpupillary

distance again as required (also see section 2.4).

• The aperture diaphragm (3-2/4) must be set to

approx. half the size of the opening diameter

by turning the adjustment wheel downwards.

• Pull out pushrod for luminous-field diaphragm

(3-2/5) until stop.

• Use the coarse/fine drive (3-2/9, 8) focus the

circular luminous-field diaphragm now visible in

the field of view.

• Insert the pushrod for the luminous-field

diaphragm (3-2/5) again; the entire field of view

is now visible.

• Refocusing on the specimen can be performed via the fine drive (3-2/8).

• Use the illuminance control (3-2/7) to set the brightness in the field of view to a value agreeable to

your eyes.

☞

Depending on the specimen, the contrast is now adjusted via the aperture diaphragm (3-2/4).

However, the aperture diaphragm cannot be used to control the image brightness (loss in

image resolution).

Fig. 3-2 Setting of reflected-light brightfield

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Zeiss Axiolab A Bedienerhandbuch Seite 46