Zeiss Axiolab A Bedienerhandbuch PDF herunterladen (Seite 55)

Axiolab A Carl Zeiss

B 40-015 e 06/99 3-13

3.2.4

Setting of Epi-Fluorescence

The fluorescence technique allows fluorescent structures to be visualized with high contrast in a typical

fluorescence color. The examined material can be autofluorescent or may have been mixed with

fluorescent substances (fluorochromes).

The epi-fluorescence technique can be performed using equipment for simple fluorescence examinations

with the Axiolab A, together with the 6 V, 30 W halogen illuminator (3-9/1) and the standard brightfield

beam splitter.

The equipment consists of:

- a special FL filter slider (3-9/2) which is inserted

in the reflected-light illuminator H instead of

the standard filter slider.

- a dia. 25 excitation filter (3-9/3) especially for

the blue excitation of Epodye.

- a dia. 25 barrier filter (3-9/5) especially matched

to this excitation filter, which is inserted in one

of the two dust protection sliders (3-9/6).

☞

This technique requires much light.

Turn the "illuminance" control (3-9/7)

to its right stop, fully open aperture

diaphragm (3-9/4) via the adjusting

wheel.

Fig. 3-9 Setting of epi-fluorescence

1 2 ... 50 51 52 53 54 55 56 57 58 59 60 ... 75 76

Keine Kommentare

Zeiss Axiolab A Bedienerhandbuch Seite 55