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Carl Zeiss Axiolab A

3-14 B 40-015 e 06/99

3.2.5

Setting of Transmitted-Light Brightfield (KÖHLER Illumination)

Make the microscope ready for operation as

described in chapter 2 and section 3.1.

• Switch on the illumination, place a high-

contrast specimen on the stage, and focus

using the 10× objective.

• Close luminous-field diaphragm (3-10/3) via the

knurled wheel.

• Use drive (3-10/5) to move ABBE condenser

close to the upper stop position until the image

of the luminous-field diaphragm appears in

focus.

• Use the centering screws (3-10/2) to move the

luminous-field diaphragm to the center of the

field of view.

• Open the luminous-field diaphragm until its

edge just disappears from the field of view.

• Control the contrast via the aperture diaphragm

(3-10/4), which should be open by approx. 2/3

of its size (marking on condenser positioned to

0.5).

For further information on the use of the

Axiolab A for transmitted-light examinations and

the relevant accessories please see the manual

Axiolab - Transmitted Light and Epi-Fluorescence

(G 42-130/II e).

Fig. 3-10 Setting of transmitted-light

brightfield

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Zeiss Axiolab A Bedienerhandbuch Seite 56